Abstract
Purpose
The purpose of this study was to determine the effects of naringin on osteoclastogenesis and osteolysis both in vitro and in vivo.
Methods
In this research osteoclasts were generated from mouse bone marrow monocytes with the receptor activator of NF-КB ligand and the macrophage colony stimulating factor. Naringin, at a concentration of 1, 10, 50, and 100 μg/mL, was respectively added to the medium. Seven days later, the osteoclasts were determined through tartrate-resistant acid phosphatase (TRAP) staining. Mature osteoclasts were isolated from newborn rabbits and cultured for three days on bone slices. Naringin at a concentration of 1, 10, 50, and 100 μg/mL was respectively added to the medium. The resorption bone slices were quantified, and the area was calculated after toluidine blue and Mayer-hematoxylin staining. Polymethyl methacrylate (PMMA) particles were implanted on the calvariae of C57BL/J6 mice. Naringin, at a dose of 50 μg/kg and 100 μg/kg, was respectively given intraperitoneally for seven. Seven days later, the calvariae were removed and processed for pathological analysis.
Results
The result indicated that naringin treatment effectively inhibited in vitro osteoclastogenesis and inhibited mature osteoclasts. In vivo data indicated that naringin strongly inhibited PMMA-induced osteolysis.
Conclusion
Naringin can effectively inhibit osteoclastogenesis and suppress wear particles-induced osteolysis and might be useful in the treatment or prevention of wear particles-induced osteolysis and aseptic loosening for its effect on osteoclast generation and function.
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Acknowledgments
This research was supported by the Shanghai Postdoctoral Sustentation Fund, China (Grant No. 11R21414400), China Postdoctoral Science Foundation (Grant No. 20100480598) and National Natural Science Foundation of China (Grant No. 81071487).
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The authors declare that they have no conflict of interest with this paper.
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Xiaowei Yu and Xinwei Zhao contributed equally as the first author.
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Yu, X., Zhao, X., Wu, T. et al. Inhibiting wear particles-induced osteolysis with naringin. International Orthopaedics (SICOT) 37, 137–143 (2013). https://doi.org/10.1007/s00264-012-1668-5
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DOI: https://doi.org/10.1007/s00264-012-1668-5