Abstract.
The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 °C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability decreases to 25% at 99 MPa. Yeast resistance to high osmotic stress (99 Mpa) is enhanced at low temperatures (5–11 °C). Therefore, the temperature at which hyperosmotic pressure is achieved greatly affects cell viability. These results suggest that temperature control is a suitable means of enhancing cell survival in response to osmotic dehydration.
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Received revision: 19 January 2001
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Beney, .L., Marechal, .P. & Gervais, .P. Coupling effects of osmotic pressure and temperature on the viability of Saccharomyces cerevisiae . Appl Microbiol Biotechnol 56, 513–516 (2001). https://doi.org/10.1007/s002530100619
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DOI: https://doi.org/10.1007/s002530100619