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Identification of critical residues for the activity and thermostability of Streptomyces sp. SK glucose isomerase

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Abstract

The role of residue 219 in the physicochemical properties of d-glucose isomerase from Streptomyces sp. SK strain (SKGI) was investigated by site-directed mutagenesis and structural studies. Mutants G219A, G219N, and G219F were generated and characterized. Comparative studies of their physicochemical properties with those of the wild-type enzyme highlighted that mutant G219A displayed increased specific activity and thermal stability compared to that of the wild-type enzyme, while for G219N and G219F, these properties were considerably decreased. A double mutant, SKGI F53L/G219A, displayed a higher optimal temperature and a higher catalytic efficiency than both the G219A mutant and the wild-type enzyme and showed a half-life time of about 150 min at 85 °C as compared to 50 min for wild-type SKGI. Crystal structures of SKGI wild-type and G219A enzymes were solved to 1.73 and 2.15 Å, respectively, and showed that the polypeptide chain folds into two structural domains. The larger domain consists of a (β/α)8 unit, and the smaller domain forms a loop of α helices. Detailed analyses of the three-dimensional structures highlighted minor but important changes in the active site region as compared to that of the wild-type enzyme leading to a displacement of both metal ions, and in particular that in site M2. The structural analyses moreover revealed how the substitution of G219 by an alanine plays a crucial role in improving the thermostability of the mutant enzyme.

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Acknowledgments

This work was funded by the Tunisian Ministry of Higher Education, the French–Tunisian CMCU programme no. 09 G 0801, and the French National Research Center (CNRS).

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Authors and Affiliations

  1. Laboratoire de Micro-organismes et de Biomolécules, Centre de Biotechnologie de Sfax, Université de Sfax, BP “1177”, 3018, Sfax, Tunisia

    Hajer Ben Hlima & Samir Bejar

  2. Laboratoire de BioCristallographie et Biologie Structurale des Cibles Thérapeutiques, Bases Moléculaires et Structurales des Systèmes Infectieux, UMR 5086-CNRS/Université Lyon 1, Institut de Biologie et Chimie des Protéines FR3302, 7 Passage du Vercors, 69367, Lyon cedex 07, France

    Jonas Riguet, Richard Haser & Nushin Aghajari

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  1. Hajer Ben Hlima
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  2. Samir Bejar
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  3. Jonas Riguet
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  4. Richard Haser
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  5. Nushin Aghajari
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Correspondence to Samir Bejar.

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Ben Hlima, H., Bejar, S., Riguet, J. et al. Identification of critical residues for the activity and thermostability of Streptomyces sp. SK glucose isomerase. Appl Microbiol Biotechnol 97, 9715–9726 (2013). https://doi.org/10.1007/s00253-013-4784-2

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