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Isolation of Iodide-Oxidizing Bacteria from Iodide-Rich Natural Gas Brines and Seawaters

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Abstract

Iodide-oxidizing bacteria (IOB), which oxidize iodide (I) to molecular iodine (I2), were isolated from iodide-rich (63 μM to 1.2 mM) natural gas brine waters collected from several locations. Agar media containing iodide and starch were prepared, and brine waters were spread directly on the media. The IOB, which appeared as purple colonies, were obtained from 28 of the 44 brine waters. The population sizes of IOB in the brines were 102 to 105 colony-forming units (CFU) mL−1. However, IOB were not detected in natural seawaters and terrestrial soils (fewer than 10 CFU mL−1 and 102 CFU g wet weight of soils−1, respectively). Interestingly, after the enrichment with 1 mM iodide, IOB were found in 6 of the 8 seawaters with population sizes of 103 to 105 CFU mL−1. 16S rDNA sequencing and phylogenetic analyses showed that the IOB strains are divided into two groups within the α-subclass of the Proteobacteria. One of the groups was phylogenetically most closely related to Roseovarius tolerans with sequence similarities between 94% and 98%. The other group was most closely related to Rhodothalassium salexigens, although the sequence similarities were relatively low (89% to 91%). The iodide-oxidizing reaction by IOB was mediated by an extracellular enzyme protein that requires oxygen. Radiotracer experiments showed that IOB produce not only I2 but also volatile organic iodine, which were identified as diiodomethane (CH2I2) and chloroiodomethane (CH2ClI). These results indicate that at least two types of IOB are distributed in the environment, and that they are preferentially isolated in environments in which iodide levels are very high. It is possible that IOB oxidize iodide in the natural environment, and they could significantly contribute to the biogeochemical cycling of iodine.

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Acknowledgments

We thank K. Redeker (Queen’s University of Belfast) for the critical reading of the manuscript; S. Yoshida (National Institute of Radiological Sciences, Japan) and G. Snyder (Rice University) for their valuable comments and kind suggestions throughout this study; K. Kimura (Chiba University), N. Machida (Chiba University), Y. Mishima (Chiba University), and S. Yamazaki (Tokyo Nuclear Service Co.) for their technical support. We are also grateful to the following iodine-producing companies for providing brine samples: Kanto Natural Gas Development Co., Nippoh Chemical Co., Godo-Shigen-Sangyo Co., Ise Chemical Co., Konoura Gas and Water Office, and Teikoku Oil Co. This work was made possible by research grants from the Forum on Iodine Utilization and the Hamaguchi Foundation for the Advancement of Biochemistry to S. A.

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Correspondence to Seigo Amachi.

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Amachi, S., Muramatsu, Y., Akiyama, Y. et al. Isolation of Iodide-Oxidizing Bacteria from Iodide-Rich Natural Gas Brines and Seawaters. Microb Ecol 49, 547–557 (2005). https://doi.org/10.1007/s00248-004-0056-0

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