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Profiling of nanoparticle–protein interactions by electrophoresis techniques

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Abstract

The use of nanomaterials in the chemical, biomedical, and biotechnological sciences is growing, increasing the possible release of these materials into the environment and contact with living organisms. Because of their large surface area, biomolecules can be adsorbed on the surface of nanomaterials. Proteins bind to the surface of nanoparticles (NPs), forming a biological layer around the NP, the “protein corona,” which gives new characteristics to the NPs in biological milieu and may affect biomedical applications or induce nanotoxicological effects. Therefore, the development of analytical tools for identification of NP protein corona behavior is essential. Techniques such as spectroscopy, chromatography, calorimetry, and electrophoresis have been used to investigate the interaction of NPs with proteins. This review describes recent developments in the application of electrophoresis techniques for profiling of NP–protein interactions. Further, we provide an overview of directions and challenges in the application of electrophoresis methods for the investigation of NP–protein structures.

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Abbreviations

C3:

Complement component 3

CD:

Circular dichroism

CE:

Capillary electrophoresis

DIGE:

Difference gel electrophoresis

ICP:

Inductively coupled plasma

ITC:

Isothermal titration calorimetry

LC:

Liquid chromatography

MS:

Mass spectrometry

MS/MS:

Tandem mass spectrometry

NMR:

Nuclear magnetic resonance

NP:

Nanoparticle

PDADMAC:

Poly(diallyldimethylammonium chloride)

PAGE:

Polyacrylamide gel electrophoresis

PSS:

Poly(sodium 4-styrenesulfonate)

SDS:

Sodium dodecyl sulfate

SILAC:

Stable isotope labeling by amino acids in cell culture

SPIO:

Superparamagnetic iron oxide

SPR:

Surface plasmon resonance

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    Mohammad Zarei & Jamal Aalaie

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Zarei, M., Aalaie, J. Profiling of nanoparticle–protein interactions by electrophoresis techniques. Anal Bioanal Chem 411, 79–96 (2019). https://doi.org/10.1007/s00216-018-1401-3

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