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Rapid monitoring of plant growth regulators in bean sprouts via automated on-line polymeric monolith solid-phase extraction coupled with liquid chromatography tandem mass spectrometry

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Abstract

An automated on-line solid-phase extraction (SPE) following liquid chromatography tandem mass spectrometry was established for the fast determination of plant growth regulator residues in soybean sprout and mung bean sprout. The crude extracted specimens were directly purified on a poly (2-(dimethylamino) ethyl methacrylate-co-ethylene dimethacrylate) monolithic column which was well-defined as the on-line SPE adsorbent. Under the optimized conditions, the developed method gave the linear range of 0.3–50 ng/mL for gibberellin and 2,4-dichlorophenoxyacetic acid, 0.2–50 ng/mL for 4-chlorophenoxyacetic acid, and 0.5–50 ng/mL for 1-naphthaleneacetic acid (r ≥ 0.998). The detection limits (S/N = 3) ranged from 1.0 to 2.5 μg/kg and the recoveries for spiked soybean sprout samples were in the range of 75.0–93.3%. Besides, the total time for one analysis was 16 min. The reusability of the monolith was up to 600 extractions. The proposed process facilitated fully automated SPE and accurate determination in one step with rapidity, simplicity, and reliability.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 21305028), the Natural Science Foundation of Hebei Province, China (No. H2017209232 and No.H2016209018), the Research Foundation of Education Bureau of Hebei Province, China (No. ZD2018014), and the Training Foundation of North China University of Science and Technology (No. JQ201717).

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Authors and Affiliations

  1. School of Public Health, North China University of Science and Technology, No. 21 Bohai Road, Caofeidian, Tangshan, 063210, Hebei, China

    Qixun Nian, Dongmei Li, Xuelei Chen, Lei Zhang, Manman Wang & Xuesheng Wang

  2. Hebei Entry-Exit Inspection and Quarantine Bureau, No. 318, Heping West Road, Shijiazhuang, 050000, Hebei, China

    Lianfeng Ai

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  1. Qixun Nian
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  4. Xuelei Chen
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Correspondence to Manman Wang.

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Nian, Q., Ai, L., Li, D. et al. Rapid monitoring of plant growth regulators in bean sprouts via automated on-line polymeric monolith solid-phase extraction coupled with liquid chromatography tandem mass spectrometry. Anal Bioanal Chem 410, 7239–7247 (2018). https://doi.org/10.1007/s00216-018-1334-x

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