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One-step synthesized flower-like materials used for sensitively detecting amyloid precursor protein

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Abstract

In this paper, we developed a new method to detect Alzheimer’s disease (AD)-related amyloid precursor protein (APP). A composite material containing horseradish peroxidase (HRP), APP antibody, and Cu3(PO4)2 was synthesized as the biosensor by co-precipitation method. In this competitive immunoassay, APP was first conjugated onto the microplate surface with the help of poly-L-lysine as the coating reagent; the composite materials were then attached onto the microplate through the interaction of APP and antibody; the HRP can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) and formed colored species. Therefore, the more APP in the detection solution (free form), the less composite material was combined with the immobilized APP on the microplate, resulting in the production of less colored TMB species. A series of detection parameters were studied, such as the composite material synthesis process, the concentration, and reaction time of different compounds. Our method has higher sensitivity compared with the similar immunoassay without using composite materials (the limits of detection are 0.3 and 3 ng/mL, respectively), and can be used for real samples (human serum) detection. The detection results using our method are consistent with the ELISA results, which is useful for the AD detection.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 21305032), China Postdoctoral Science Foundation (No. 2014M551522), Jiangsu Planned Projects for Postdoctoral Research Funds (No. 1402073B), and Hong Kong Scholar Program (No. XJ2017008).

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Authors and Affiliations

  1. College of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China

    Chengke Wang, Rong Tan & Qingqing Wang

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  1. Chengke Wang
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Correspondence to Chengke Wang.

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This is not a clinical study on humans with an ethics committee. The biological samples were transferred to our laboratory from the Affiliated Hospital of Jiangsu University. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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The authors declare that they have no competing interests

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Wang, C., Tan, R. & Wang, Q. One-step synthesized flower-like materials used for sensitively detecting amyloid precursor protein. Anal Bioanal Chem 410, 6901–6909 (2018). https://doi.org/10.1007/s00216-018-1293-2

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  • DOI: https://doi.org/10.1007/s00216-018-1293-2

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