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Synthesis and application of ratio fluorescence probe for chloride

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Abstract

As chloride ions (Cl−) play a vital role in maintaining normal physiological activity, detection of chloride ions is quite urgent. Hence, we developed chloride fluorescence probes to highly selectively and sensitively monitor chloride ions. The probe M2 with single emission has a high fluorescence quantum yield (Φ = 45%), and it is capable of quantitative detection of Cl− under physiological conditions (pH = 7.4) and pH = 5.0 with a linear range of 0.1–4.0 mM; nevertheless, it is of the switch-off type. We further synthesized a ratiometric fluorescent probe MY with M2 as raw material, which featured excellent selectivity and anti-interference, and large two-photon cross section (555 GM). The probe is conveniently used to detect Cl− in water samples and biological samples including human sweat, serum, and urine samples, indicating it holds great promise for chloride detection and biological application.

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Funding

This research was financially supported by the National Natural Science Foundation of China (No. 21575055) and the Research Funds for the Central Universities (lzujbky-2017-k09).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, Gansu, China

    Chen Ma, Fengyuan Zhang, Yaya Wang, Xinyue Zhu, Xiaoyan Liu & Haixia Zhang

  2. School of Pharmacy, Lanzhou University, Lanzhou, 730000, Gansu, China

    Chunyan Zhao

Authors
  1. Chen Ma
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  2. Fengyuan Zhang
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  3. Yaya Wang
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  4. Xinyue Zhu
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  5. Xiaoyan Liu
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  6. Chunyan Zhao
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  7. Haixia Zhang
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Corresponding author

Correspondence to Haixia Zhang.

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The study using biological samples has been approved by the People’s hospital of Gansu province Ethics Committee and the Lanzhou University Ethics Committee, and has been performed in accordance with the ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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The authors declare that they have no conflict of interest.

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Ma, C., Zhang, F., Wang, Y. et al. Synthesis and application of ratio fluorescence probe for chloride. Anal Bioanal Chem 410, 6507–6516 (2018). https://doi.org/10.1007/s00216-018-1250-0

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  • DOI: https://doi.org/10.1007/s00216-018-1250-0

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