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Multiclass screening of >200 pharmaceutical and other residues in aquatic foods by ultrahigh-performance liquid chromatography–quadrupole-Orbitrap mass spectrometry

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Abstract

A quick screening method of more than 200 pharmaceutical and other residues in aquatic foods based on ultrahigh-performance liquid chromatography–quadrupole-Orbitrap mass spectrometry (UHPLC-Q/Orbitrap MS) was established. In this method, after the addition of 200 μL of 1 M EDTA-Na2, 2 g of each sample homogenate was extracted successively with 10 mL of acetonitrile and 10 mL of ethyl acetate. The extracts were combined, dried under nitrogen flow, and redissolved in 0.1% formic acid in acetonitrile/water (4:6, v/v) for analysis. The prepared samples were analyzed by UHPLC- Q/Orbitrap MS system in Full MS/ddMS2 (full-scan data-dependent MS/MS) mode. Compound identification was performed through comparison of the sample data with the database for standard chemicals, including the retention time, precursor ion, product ions, and isotope pattern for all 206 compounds. Five different aquatic food matrices (carp, shrimp, crab, eel, and mussel) spiked with the analytes at 1, 10, and 50 ng/g were evaluated to assess recoveries, precision, matrix effects, stability, and detection limits using the method. UHPLC analyses required 25 min, and 178–200 analytes met identification criteria at 50 ng/g depending on the matrix. Furthermore, practical application of this method for real samples displayed strong screening capability.

A quick screening method of >200 pharmaceutical and other residues in aquatic foods based on ultrahighperformance liquid chromatography–quadrupole-Orbitrap mass spectrometer was established. Fivedifferent aquatic food matrices, including carp, shrimp, crab, eel and mussel, were studied to evaluatescreen limit at 1, 10 and 50 μg·kg-1 level. Results suggest the high reliability, high time-efficiency and goodsimplicity of the method.

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Acknowledgments

The authors would like to acknowledge the financial support from the Shanghai Municipal Agricultural Commission, the Yangfan project (14YF1408100) from Science and Technology Commission of Shanghai Municipality – PR China and the special research fund for the national non-profit institutes (East China Sea Fisheries Research Institute) (No. 2014T05).

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Authors and Affiliations

  1. Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, 200090, China

    Cong Kong, Yang Wang & Yuanfei Huang

  2. College of Food Science & Technology, Shanghai Ocean University, Shanghai, 201306, China

    Yang Wang & Yuanfei Huang

  3. Key Laboratory of Oceanic and Polar Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, 200090, China

    Huijuan Yu

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  1. Cong Kong
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Correspondence to Cong Kong or Huijuan Yu.

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Published in the topical collection Food Safety Analysis with guest editor Steven J. Lehotay.

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Kong, C., Wang, Y., Huang, Y. et al. Multiclass screening of >200 pharmaceutical and other residues in aquatic foods by ultrahigh-performance liquid chromatography–quadrupole-Orbitrap mass spectrometry. Anal Bioanal Chem 410, 5545–5553 (2018). https://doi.org/10.1007/s00216-018-1124-5

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  • DOI: https://doi.org/10.1007/s00216-018-1124-5

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