Abstract
Here, we utilized the ultrasonic emulsification technique to generate hyaluronic acid microspheres incorporating a fluorescence-based glucose biosensor. We synthesized a novel lanthanide ion luminophore based on Eu3+. Eu sulfosuccinimidyl dextran (Eu-dextran) and Alexa Fluor 647 sulfosuccinimidyl-ConA (Alexa Fluor 647-ConA) were encapsulated in hyaluronic acid hydrogel to generate microspheres. Glucose sensing was carried out using a fluorescence resonance energy transfer (FRET)-based assay principle. A proportional fluorescence intensity increase was found within a 0.5–10-mM glucose concentration range. The glucose-sensing strategy showed an excellent tolerance for potential interferents. Meanwhile, the fluorescent signal of hyaluronic acid microspheres was very stable after testing for 72 h in glucose solution. Overall, hyaluronic acid microspheres encapsulating sensing biomolecules offer a stable and biocompatible biosensor for a variety of applications including cell culture systems, tissue engineering, detection of blood glucose, etc.
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The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was funded and supported by grants from the National Natural Science Foundation of China (No. 21307154), National Post-Doctor Science Foundation of China (No. 2017M621825), Post-Doctor Foundation of Jiangsu Province (No. 1701034C), and Industry-University Collaboration Project of Jiangsu Province (No. BY2015040-01).
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Ge, M., Bai, P., Chen, M. et al. Utilizing hyaluronic acid as a versatile platform for fluorescence resonance energy transfer-based glucose sensing. Anal Bioanal Chem 410, 2413–2421 (2018). https://doi.org/10.1007/s00216-018-0928-7
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DOI: https://doi.org/10.1007/s00216-018-0928-7