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Multiplex highly sensitive immunochromatographic assay based on the use of nonprocessed antisera

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Abstract

The format of an immunochromatographic multiassay is first proposed with native antisera and a universal conjugate of antispecies antibodies with gold nanoparticles. This format allows (1) the exclusion of purification and conjugation stages for specific antibodies and (2) significant reduction of the concentration of specific antibodies in the system. The independent use of specific antibodies and a conjugated marker provided a low detection limit and high signal intensity. The proposed format was implemented for the simultaneous detection of two herbicides. The instrumental limits for the detection of atrazine and chlorsulfuron were 0.1 and 0.7 ng/mL, respectively, and the analysis time was 20 min. The suitability of the test system for monitoring these herbicides in nontreated apple and blackcurrant juices is shown. The assay technique is simple, sensitive, and easily transferrable to any other antigen.

The proposed format of the immunochromatographic multiassay is based on the use of native antisera and a universal conjugate of antispecies antibodies with gold nanoparticles. In this way purification and conjugation stages for specific antibodies are excluded, and the concentrations of specific antibodies and the conjugated marker can be varied independently to obtain a low detection limit.

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Acknowledgements

The authors are thankful to S.M. Pridvorova (A.N. Bach Institute of Biochemistry) for the transmission electron microscopy measurements and data processing. This research was financially supported by the Russian Science Foundation (grant 14-16-00149).

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Authors and Affiliations

  1. A.N. Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology”, Leninsky prospeсt 33-2, 119071, Moscow, Russia

    Nadezhda A. Byzova, Alexandr E. Urusov, Anatoly V. Zherdev & Boris B. Dzantiev

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  1. Nadezhda A. Byzova
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  2. Alexandr E. Urusov
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  3. Anatoly V. Zherdev
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  4. Boris B. Dzantiev
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Correspondence to Boris B. Dzantiev.

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Byzova, N.A., Urusov, A.E., Zherdev, A.V. et al. Multiplex highly sensitive immunochromatographic assay based on the use of nonprocessed antisera. Anal Bioanal Chem 410, 1903–1910 (2018). https://doi.org/10.1007/s00216-018-0853-9

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