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A sensitive immunosorbent bio-barcode assay based on real-time immuno-PCR for detecting 3,4,3',4'-tetrachlorobiphenyl

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Abstract

A functionalized gold-nanoparticle bio-barcode assay, based on real-time immuno-PCR (IPCR), was designed for the determination of 3,4,3',4'-tetrachlorobiphenyl (PCB77). 15 nm gold nanoparticles were synthesized, and modified with thiol-capped DNA and goat anti-rabbit IgG. The nanoparticle probes were used to replace antibody–DNA conjugate in the IPCR, and were fixed on the PCR tube wall via the immune reaction. Real-time PCR was performed to quantify the DNA signal directly. Under optimized conditions, the new method was used to detect PCB77 with a linearity range from 5 pg L−1 to 10 ng L−1, and the limit of detection (LOD) was 1.72 pg L−1. Real samples of Larimichthys polyactis, collected from the East China Sea, were analyzed. Recovery was from 82 % to 112 %, and the coefficient of variation (CV) was acceptable. The results were compared with GC–ECD, revealing that the method would be acceptable for providing rapid, semi-quantitative, and reliable test results for making environmental decisions.

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Acknowledgements

We gratefully acknowledge the National Natural Science Foundation of China (Project No. 21177082), the Science and Technology Commission of Shanghai Municipality in China (Key Project of Fundamental Research No. 09JC1407600), Shanghai Jiaotong University science and technology innovation special fund development projects, and Shanghai Jiao Tong University Innovation Fund for Postgraduates for their financial support.

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  1. School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China

    Guang-Xin Yang, Hui-Sheng Zhuang, Han-Yu Chen, Xian-Yin Ping & Dan Bu

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  1. Guang-Xin Yang
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  2. Hui-Sheng Zhuang
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  4. Xian-Yin Ping
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Correspondence to Hui-Sheng Zhuang.

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Yang, GX., Zhuang, HS., Chen, HY. et al. A sensitive immunosorbent bio-barcode assay based on real-time immuno-PCR for detecting 3,4,3',4'-tetrachlorobiphenyl. Anal Bioanal Chem 406, 1693–1700 (2014). https://doi.org/10.1007/s00216-013-7583-9

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  • DOI: https://doi.org/10.1007/s00216-013-7583-9

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