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Regulation of the deleterious effects of binge-like exposure to alcohol during adolescence by α7 nicotinic acetylcholine receptor agents: prevention by pretreatment with a α7 negative allosteric modulator and emulation by a α7 agonist in alcohol-preferring (P) male and female rats

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Abstract

Rationale and objectives

Binge-like alcohol consumption during adolescence associates with several deleterious consequences during adulthood including an increased risk for developing alcohol use disorder (AUD) and other addictions. Replicated preclinical data has indicated that adolescent exposure to binge-like levels of alcohol results in a reduction of choline acetyltransferase (ChAT) and an upregulation in the α7 nicotinic receptor (α7). From this information, we hypothesized that the α7 plays a critical role in mediating the effects of adolescent alcohol exposure.

Methods

Male and female P rats were injected with the α7 agonist AR-R17779 (AR) once during 6 time points between post-natal days (PND) 29–37. Separate groups were injected with the α7 negative allosteric modulator (NAM) dehydronorketamine (DHNK) 2 h before administration of 4 g/kg EtOH (14 total exposures) during PND 28–48. On PND 75, all rats were given access to water and ethanol (15 and 30%) for 6 consecutive weeks (acquisition). All rats were then deprived of EtOH for 2 weeks and then, alcohol was returned (relapse).

Results

Administration of AR during adolescence significantly increased acquisition of alcohol consumption during adulthood and prolonged relapse drinking in P rats. In contrast, administration of DHNK prior to binge-like EtOH exposure during adolescence prevented the increase in alcohol consumption observed during acquisition of alcohol consumption and the enhancement of relapse drinking observed during adulthood.

Discussion

The data indicate that α7 mediates the effects of alcohol during adolescence. The data also indicate that α7 NAMs are potential prophylactic agents to reduce the deleterious effects of adolescent alcohol abuse.

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Funding

The researchers were supported by NIAAA grants AA07611, AA07462, AA10721, AA20908, and AA019366 and NIA grant (R01AG051086). Research funds to conduct the experiments were provided by the Department of Psychiatry, Indiana University School of Medicine (chair: Thomas W. McAllister).

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Correspondence to Zachary A. Rodd.

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DKL is a member of the advisory boards for Entia Biosciences, Drug Discovery and Therapy World Congress, and Provaidya LLC. He also has stock options from QR Pharma for patents or patents pending on AIT-082, Memantine, Acamprosate, and GILZ analogs. DKL is the Editor-in-Chief of the journal “Current Alzheimer Research.” DKL also had prior funding from Baxter and Forest Research Labs. All have no direct influence on the research presented here. Finally, DKL declares no other actual or potential competing interests in the subject matter of this paper. ZAR, SRH, RAW, and RLB have used the current data set to apply for a provisional patent titled “The use of alpha-7 nicotinic receptor negative allosteric modulators for the prevention of adolescent alcohol consumption producing alcohol use disorder (AUD) and drug addiction during adulthood” with the US and EU patent office.

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Rodd, Z.A., Hauser, S.R., Swartzwelder, H.S. et al. Regulation of the deleterious effects of binge-like exposure to alcohol during adolescence by α7 nicotinic acetylcholine receptor agents: prevention by pretreatment with a α7 negative allosteric modulator and emulation by a α7 agonist in alcohol-preferring (P) male and female rats. Psychopharmacology 237, 2601–2611 (2020). https://doi.org/10.1007/s00213-020-05557-1

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