Abstract
Bacillus thuringiensis (Bt) is efficient, strongly specific, and avirulent to humans, making it one of the most popular biopesticides in the world. Bt LLP29 is a mosquitocidal strain that was first isolated from Magnolia denudata. To understand its molecular mechanism against mosquitoes, the genome of Bt LLP29 was sequenced and annotated in this study. The LLP29 genome was found to have a total length of 5.99 Mb, with an average G + C content of 35.21%. A total of 6107 coding sequences were also detected, together with 42 rRNAs and 124 tRNAs and 135 other RNAs. With the help of annotation databases, including GO, COG, KEGG, Nr and Swiss-Prot, most unigene functions were identified. At the same time, a collinear analysis was performed on the genome of LLP29. There were also some virulence genes detected, including cry, chitinase, zwittermicin and vip.
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NCBI gene bank https://www.ncbi.nlm.nih.gov/bioproject/PRJNA399840.
Abbreviations
- Bt:
-
Bacillus thuringiensis
- ICP:
-
Insecticidal crystalline proteins
- NCBI:
-
National Center for Biotechnology Information
- BLAST:
-
Basic Local Alignment Search Tool
- UV:
-
Ultraviolet
- GO:
-
Gene ontology
- COG:
-
Clusters of orthologous groups of proteins
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
- SAM:
-
A kind of Map Samtools
- NR:
-
Non-redundant protein sequence database
- PIR:
-
Protein identification resource
- PR:
-
Protein resource
- PDB:
-
Protein data bank
- BWA:
-
Burrows–Wheeler alignment tool
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Acknowledgements
The authors thank Mr. Alexander C. Barton from United Kingdom for language correction and additional assistance in improving the text.
Funding
This work was supported by the National Program of China (Grant Number YS2017YFGH000833 and 2017YFD0200400); the United Fujian Provincial Health and Education Project for Tackling Key Research (Grant No. 2019-WJ-29); the Foreign Cooperative Project of Fujian Province (Grant Number 2018I0023); Science and Technology Project of Fuzhou (Grant Number 2018-G-70); Open Project Funds of State Key Laboratory of Pathogen and Biosecurity (Grant Number SKLPBS1838) and by institutional support RVO: 60077344 of the Biology Centre CAS, Institute of Entomology, Czech Republic.
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WM, HC, XJ, JW, IG, XG and LZ contributed equally to the study design, collection of data, development of the sampling, analyses, interpretation of results, and preparation of the paper. All authors read and approved the final manuscript.
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Ma, W., Chen, H., Jiang, X. et al. Whole genome sequence analysis of the mosquitocidal Bacillus thuringiensis LLP29. Arch Microbiol 202, 1693–1700 (2020). https://doi.org/10.1007/s00203-020-01875-2
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DOI: https://doi.org/10.1007/s00203-020-01875-2