Skip to main content
Log in

Flavobacterium nackdongense sp. nov., a cellulose-degrading bacterium isolated from sediment

  • Original Paper
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea. Optimal growth occurred at pH 7.0, 20 °C, and 0% NaCl. Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%). The DNA G+C content of strain GS13T was 36.2 mol%. The dominant fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C15:0. The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids, three unidentified lipids, and one unidentified aminophospholipid. The predominant respiratory quinone was MK-6. Our data demonstrate that strain GS13T can be distinguished from closely-related Flavobacterium species. Thus, strain GS13T is a novel Flavobacterium species, and we propose the name Flavobacterium nackdongense sp. nov. The type strain is GS13T (=KCTC 62569T = JCM 32765T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Bernardet JF, Nakagawa Y, Holmes B (2002) Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070

    CAS  PubMed  Google Scholar 

  • Béguin P, Aubert J-P (1994) The biological degradation of cellulose. FEMS Microbiol Rev 13:25–58

    Article  Google Scholar 

  • Elberson MA, Malekzadeh F, Yazdi MT, Kameranpour N, Noori-Daloii MR, Matte MH, Shahamat M, Colwell RR, Sowers KR (2000) Cellulomonas persica sp. nov. and Cellulomonas iranensis sp. nov., mesophilic cellulose-degrading bacteria isolated from forest soils. Int J Syst Evol Microbiol 50(3):993–996

    Article  CAS  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Nol Evol 17:368–376

    Article  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Joung Y, Kim H, Ahn TS, Joh K (2012) Flavobacterium yonginense sp. nov. and Flavobacterium myungsuense sp. nov., isolated from a mesotrophic artificial lake. Int J Syst Evol Microbiol 62:806–810

    Article  CAS  Google Scholar 

  • Klemm D, Heublein B, Fink H, Bohn A (2005) Cellulose: fascinating biopolymer and sustainable raw material. Angew Chem Int Ed 44:3358–3393

    Article  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  • Lee SH, Kim JM, Lee JR, Park W, Jeon CO (2010) Flavobacterium fluvii sp. nov., isolated from stream sediment. Int J Syst Evol Microbiol 60:353–357

    Article  CAS  Google Scholar 

  • Lee Y, Jeon CO (2018) Flavobacterium alvei sp. nov., isolated from a freshwater river. Int J Syst Evol Microbiol 68:1919–1924

    Article  CAS  Google Scholar 

  • Minnikin DE, Patel PV, Alshamaony L, Goodfellow M (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117

    Article  CAS  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Nam GG, Joung Y, Park M, Kim S, Jeon HT, Cho JC (2017) Flavobacterium soyangense sp.nov., a psychrotolerant bacterium, isolated from an oligotrophic freshwater lake. Int J Syst Evol Microbiol 67:2440–2445

    Article  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  • Schwarz WH (2001) The cellulosome and cellulose degradation by anaerobic bacteria. Appl Microbiol Biotechnol 56:634–649

    Article  CAS  Google Scholar 

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654

  • Yoon JH, Choi JH, Kang SJ, Choi NS, Lee JS, Song JJ (2010) Jeongeupia naejangsanensis gen. nov., sp. nov., a cellulose-degrading bacterium isolated from forest soil from Naejang Mountain in Korea. Int J Syst Evol Microbiol 60:615–619

    Article  CAS  Google Scholar 

  • Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We are grateful to Dr. Aharon Oren for his advice on naming this microorganism.

Funding

This work was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NNIBR201901108).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Hyangmi Kim or Sang Mi Yu.

Additional information

Communicated by Erko Stackebrandt.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and genome sequences of strain GS13T are MH168565 and CP037933, respectively.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 12 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kim, H., Yu, S.M. Flavobacterium nackdongense sp. nov., a cellulose-degrading bacterium isolated from sediment. Arch Microbiol 202, 591–595 (2020). https://doi.org/10.1007/s00203-019-01770-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-019-01770-5

Keywords

Navigation