Abstract
A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterium, designated HYT19T, was isolated from soil of Mountain Danxia in southern China. It showed the highest similarity of 16S rRNA gene sequence (97.0%) and formed a monophyletic clade with Fibrisoma limi BUZ 3T. Strain HYT19T grew at 16–37 °C (optimum 28–30 °C) and at pH 6–7. The draft genome size of strain HYT19T was 7.8 Mb with a DNA G+C content of 54.0 mol%. The digital DDH and average nucleotide identity values between strain HYT19T and F. limi BUZ 3T were 28.8% and 85.1%, respectively. MK-7 was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, unidentified aminophospholipid, two unidentified aminolipids, unidentified phospholipid and unidentified lipid. The strain contained C16:1ω5c, iso-C15:0, summed feature 3 (C16:1ω6c and/or C16:1ω7c), C16:0, iso-C17:0 3-OH and anteiso-C15:0 as the major fatty acids. On the basis of phylogenetic, genomic, phenotypic and chemotaxonomic analysis, we propose a new species Fibrisoma montanum sp. nov. of genus Fibrisoma. The type strain is HYT19T (= CCTCC AB 2018342T = JCM 33105T).
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Acknowledgements
We are grateful to Prof. Bernhard Schink for the Latin construction of the species name. Fatty acid and quinone analyses were performed by the China Center for Type Culture Collection (CCTCC). Polar lipid analyses were performed by Guangdong Detection Centre of Microbiology, P. R. China. This work was supported by the National Natural Science Foundation of China (No. 41771283).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Fibrisoma montanum HYT19T is MH989567. The Draft Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession QXED00000000. The version described in this paper is version QXED01000000. The digital protologue database (DPD) Taxon Number of strain HYT19T is CA00064.
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Huang, Y., Niu, Y., Wang, G. et al. Fibrisoma montanum sp. nov., isolated from soil of Mountain Danxia, China. Arch Microbiol 202, 269–273 (2020). https://doi.org/10.1007/s00203-019-01741-w
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DOI: https://doi.org/10.1007/s00203-019-01741-w