Abstract
Plant endophytes play a crucial role in plant growth, health and ecological function. Jingbai pear (the best quality cultivar of Pyrus ussuriensi Maxim. ex Rupr.) has important ecological and economic value in north China. Conversation of its genetics has great meanings to pear genus (Pyrus L.). However, the bacterial community associated with the cultivar remains unknown. In this study, the structure of endophytic bacterial communities associated with different tissues and soil of Jingbai Pear trees was analyzed with Illumina Miseq sequencing of bacterial 16S rDNA. This is the first report on bacterial microbiome associated with Jingbai pear. Our results demonstrated that different tissues harbored a unique bacterial assemblage. Interestingly, Cyanobacteria was the most abundant phylum, followed by Proteobacteria and Actinobacteria. Samples from three different sites (soils) had significant differences in microbial communities structure. Redundancy analysis (RDA) showed that the bacterial community structure correlated significantly with soil properties—temperature, pH, nitrogen and carbon contents. The conclusion could facilitate to understand the interaction and ecological function of endophytic bacteria with host Jingbai pear trees, so as to benefit the pear variety genetic resource conservation and protection.
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The Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry is gratefully acknowledged for research funding (Project CAFYBB2017MA019).
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FR, DHY conceived the study and contributed in the experimental design of the study; FR, WD and DHY collected the samples; FR did the experiment; FR, WD performed the statistical analysis; FR wrote the first draft of the manuscript; All authors contributed to manuscript revision, read and approved the submitted version.
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Communicated by Erko Stackebrandt.
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Supplementary Figure S2. Statistically significant differences in the bacterial species richness (a), diversity (b) and evenness (c) of the samples. (TIF 82 KB)
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Supplementary Figure S3. Significantly different taxa among samples (a) phylum, (b) class, (c) family, (d) genus (e) OTUs. (TIF 311 KB)
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Ren, F., Dong, W. & Yan, DH. Endophytic bacterial communities of Jingbai Pear trees in north China analyzed with Illumina sequencing of 16S rDNA. Arch Microbiol 201, 199–208 (2019). https://doi.org/10.1007/s00203-018-1597-9
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DOI: https://doi.org/10.1007/s00203-018-1597-9