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Flavobacterium dasani sp. nov., a psychrotolerant bacterium isolated from Arctic soil

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Abstract

A novel yellow-colored, Gram-stain-negative, aerobic, non-motile, catalase- and oxidase-positive, and rod-shaped psychrotolerant bacterium, designated strain PLR-18-3T, was isolated from Arctic soil and was subjected to polyphasic taxonomic study. Cells were able to grow at 0–30 °C, pH 6.0–10.5, and 0–3.0% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, this Arctic strain belonged to the genus Flavobacterium, with the closest neighbor being Flavobacterium noncentrifugens R-HLS-17T (96.2% sequence similarity). The strain contained MK-6 as a sole respiratory quinone, phosphatidylethanolamine as the major polar lipid, and summed feature 3 (C16:1ω7c and/or C16:1ω6c), iso-C15:0, iso-C15:0 G, iso-C17:0 3-OH, iso-C15:0 3-OH, anteiso-C15:0, and summed feature 9 (iso-C17:1ω9c and/or C16:010-methyl) as the predominant fatty acids. The DNA G + C content was 37.9 mol%. On the basis of polyphasic data, strain PLR-18-3T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium dasani sp. nov. is proposed. The type strain is PLR-18-3T (=KEMB 9005-713T=KACC 19627T=NBRC 113347T).

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Abbreviations

KEMB:

Korea Environmental Microorganisms Bank

KACC:

Korean Agricultural Culture Collection

CCUG:

Culture Collection University of Gothenburg

NBRC:

NITE Biological Resource Center

LPSN:

List of Prokaryotic names with Standing in Nomenclature

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Acknowledgements

We greatly appreciate Professor Bernhard Schink (University of Konstanz, Konstanz, Germany) for providing the species name.

Funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1A09916982).

Author information

Authors and Affiliations

  1. Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do, 16227, South Korea

    Dhiraj Kumar Chaudhary & Jaisoo Kim

  2. Division of Polar Life Sciences, Polar Research Institute, Inchon, 21990, South Korea

    Dockyu Kim

Authors
  1. Dhiraj Kumar Chaudhary
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  2. Dockyu Kim
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  3. Jaisoo Kim
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Corresponding author

Correspondence to Jaisoo Kim.

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The authors declare that there is no conflict of interest.

Ethical statement

This study does not describe any experimental work related to human.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain PLR-18-3T is MH019224.

The Digital Protologue database TaxonNumber for strain PLR-18-3T is TA00585.

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Chaudhary, D.K., Kim, D. & Kim, J. Flavobacterium dasani sp. nov., a psychrotolerant bacterium isolated from Arctic soil. Arch Microbiol 201, 81–86 (2019). https://doi.org/10.1007/s00203-018-1574-3

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  • DOI: https://doi.org/10.1007/s00203-018-1574-3

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