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Bhargavaea changchunensis sp. nov. isolated from soil in China

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Abstract

A Gram-staining-positive, aerobic, rod-shaped (201802YP6T) bacteria was isolated from soil, Northeast of China. Growth occurred at 10–40 °C (optimum 25–30 °C), at pH 6.0–8.0 (optimum 7.0) and at 0–2% NaCl. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbors of strain 201802YP6T were identified as Bhargavaea cecembensis DSE10T (99.52%), Bhargavaea beijingensis ge10T (99.45%), Bhargavaea indica KJW98T (99.45%), Bhargavaea ullalensis ZMA19T (98.81%), and Bhargavaea ginsengi ge14T (98.76%). Levels of similarity among strain 201802YP6T and other Bhargavaea species were lower than 98.76%. GyrB amino acid sequence-based analysis supported the phylogenetic position and also distinguished strain 201802YP6T from the other species of the genus Bhargavaea. DNA–DNA hybridization values between strain 201802YP6T and B. cecembensis, B. beijingensis, B. indica, B. ullalensis, B. ginsengi were 43.5%, 43%, 32.5%, 30.5% and 20.4%, respectively. The DNA G + C content of strain 201802YP6T was 51.23 mol%. The average nucleotide identity (ANI) of the draft genome was 87.04% to B. cecembensis DSE10T. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipids, phosphatidylethanolamine, and phosphatidyllipid. The predominant menaquinone was MK-8. The major fatty acids were iso-C15:0 (39.91%), anteiso-C15:0 (28.86%), anteiso-C17:0 (6.30%) and C16:0 (6.13%). On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain 201802YP6T represents a novel species of the genus Bhargavaea, for which the name Bhargavaea changchunensis sp. nov. is proposed. The type strain is 201802YP6T (= CGMCC 1.13508T = KCTC 33975T).

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Acknowledgements

This work was supported by National Key Research and Development Program of China (No. 2017YFD0601002), University S & T Innovation Platform of Jilin Province for Economic Fungi (No. #2014B-1) and Special Fund for Agro-scientific Research in the Public Interest (No. 201503137).

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Authors and Affiliations

  1. Engineering Research Center of Edible and Medicinal Fungi, Jilin Agricultural University, Ministry of Education, Changchun, 130118, People’s Republic of China

    Feng-Hua Tian, Dong-Yu Fan, Chen Zhang, Chuan-Wen Jia, Yu Li & Chang-Tian Li

  2. Tonghua Edible Fungus Cultivation Cooperatives, Tonghua, 134100, People’s Republic of China

    Wei Gao

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  1. Feng-Hua Tian
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  2. Dong-Yu Fan
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  3. Chen Zhang
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  4. Chuan-Wen Jia
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  5. Wei Gao
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  6. Yu Li
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  7. Chang-Tian Li
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Correspondence to Yu Li or Chang-Tian Li.

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The authors declare that there are no conflicts of interest.

Ethical statement

This study does not describe any experimental work related to humans.

Additional information

Communicated by Erko Stackebrandt.

The NCBI GenBank accession numbers for the 16S rRNA and genome sequence of strain 201802YP6T are MH094655 and SUB4396199 (submission), respectively.

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Tian, FH., Fan, DY., Zhang, C. et al. Bhargavaea changchunensis sp. nov. isolated from soil in China. Arch Microbiol 200, 1465–1470 (2018). https://doi.org/10.1007/s00203-018-1563-6

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  • DOI: https://doi.org/10.1007/s00203-018-1563-6

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