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Interactive effects of Magnaporthe inoculation and nitrogen doses on the plant enzyme machinery and phyllosphere microbiome of resistant and susceptible rice cultivars

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Abstract

Severity of plant diseases is often influenced by the availability of nutrients, particularly N; however, its effect on the phyllosphere microbiome in foliar pathogen challenged plants is less investigated in rice. The tripartite interaction among the fungal pathogen (Magnaporthe oryzae), rice cultivars (basmati and non-basmati, blast resistant or susceptible) and nitrogen (N) fertilization (0, 120 and 180 N) was investigated. Plant growth, elicitation of defense responses and abundance of microbial members in the rice phyllosphere were monitored using biochemical and molecular methods. In general, photosynthetic pigments were distinct for each cultivar, and optimal N doses led to higher values. The susceptible var. CO-39 and resistant CO-39I exhibited higher contents of photosynthetic pigments and micronutrients such as zinc in leaves in response to N doses. Elicitation of defense and hydrolytic enzymes was significantly influenced by pathogen inoculation and modulated by N doses, but varietal effects were distinct. Scoring indices emphasized the pathogen susceptibility of var. CO-39 and PB-1, which showed almost 40–60% higher values than the resistant cultivars; the interactions of cultivars and N doses was also significant. Characteristic changes were recorded in the abundances of the gene copies, particularly, with an overall increase in the number of cyanobacterial 16S rRNA, and bacterial amoA in pathogen-challenged treatments, while nifH gene copies exhibited a reducing trend with increasing N doses, in the presence or absence of pathogen. The varietal differences in the cause and effect relationships can be valuable in crop protection for more effective foliar application of pesticides or biocontrol agents.

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Acknowledgements

The present study was partially supported by the ICAR-Network Project “Application of Microorganisms in Agricultural and Allied Sectors” (AMAAS), granted by Indian Council of Agricultural Research (ICAR), New Delhi to RP. We are thankful to the Post Graduate School, ICAR-IARI, New Delhi for the fellowship to the first author. We gratefully acknowledge the support and facilities provided by the Division of Microbiology and Division of Agronomy, ICAR-IARI, New Delhi during this study. We thank Dr. Gopalakrishnan, Division of Genetics, ICAR-IARI, New Delhi for providing the seeds of the four rice cultivars used in this study. We are thankful to Shri Gulab Singh, Dr. Y.S. Shivay and Dr. Dinesh Kumar for facilitating the analyses of macro- and micro-nutrients in the Division of Agronomy, ICAR-IARI, New Delhi.

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Concept and design of the study: ST, RP, BRK; acquisition and statistical analysis of data: ST, KV, NS, RP; drafting the manuscript and critical interpretation of the data: ST, RP, BRK, AK; supervision and critical inputs for experimental set-up and its maintenance: RP, AK. All the authors contributed to the preliminary data analyses and provided edits to the drafts of manuscript submitted.

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Correspondence to Radha Prasanna.

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Communicated by Jorge Membrillo-Hernández.

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203_2018_1540_MOESM1_ESM.pptx

Supplementary Fig. 1 Comparison of the disease severity among the cultivars, after pathogen challenge by inoculation with Magnaporthe oryzae and application of different doses of N. Arrows in the first illustration indicate the characteristic symptoms of blast pathogen - the appearance of typical water soaked lesions on the leaf surface, leading to necrosis (PPTX 1148 KB)

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Thapa, S., Prasanna, R., Ramakrishnan, B. et al. Interactive effects of Magnaporthe inoculation and nitrogen doses on the plant enzyme machinery and phyllosphere microbiome of resistant and susceptible rice cultivars. Arch Microbiol 200, 1287–1305 (2018). https://doi.org/10.1007/s00203-018-1540-0

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