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Paenibacillus panacihumi sp. nov., a potential plant growth-promoting bacterium isolated from ginseng-cultivated soil

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Abstract

A novel bacterium, designated DCY114T, was isolated from ginseng-cultivated soil in Gochang-gun, Republic of Korea. This isolate was assigned to the genus Paenibacillus and is closely related to Paenibacillus amylolyticus NRRL NRS-290T (98.3%), P. dongdonensis KUDC0114T (98.0%), P. tylopili MK2T (97.9%), P. tundrae A10bT (97.8%), and P. xylanexedens B22aT (97.5%) based on 16S rRNA gene sequence analysis. Strain DCY114T is a Gram-reaction positive, catalase and oxidase positive, facultatively aerobic rod that is motile by peritrichous flagella. Strain DCY114T produces siderophores and indole-3-acetic acid (IAA) and is able to solubilize phosphate as a plant growth-promoting bacterium. MK-7 was the diagnostic menaquinone. The major cellular fatty acids were anteiso-C15:0, C16:0, and C18:0, and the major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and an unknown amino lipid (AL1,2). The genomic DNA G + C content was 46.0 mol%. Phenotypic and chemotaxonomic results also placed strain DCY114T within the genus Paenibacillus. DNA–DNA homology values between strain DCY114T and closely related reference strains were lower than 43%. The low DNA relatedness data in combination with phylogenetic and biochemical tests indicated that strain DCY114T could not be assigned to a recognized species. The results of this study support that the DCY114T strain is a novel species belonging to the genus Paenibacillus, for which the name Paenibacillus panacihumi is proposed. The type strain is DCY114T (= KCTC 33915T = JCM 32073T).

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Acknowledgements

This study was supported by a Grant from the Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries (KIPET NO: 317007-3), Republic of Korea.

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Author notes

  1. Chun-Gon Kim and Jong-Pyo Kang are contributed equally to this work.

Authors and Affiliations

  1. Department of Oriental Medicinal Biotechnology, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea

    Chun-Gon Kim, Jong-Pyo Kang, Yue Huo, Mohan Chokkalingam, Yeon-Ju Kim, Dong-Hyun Kim & Deok-Chun Yang

  2. Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea

    Deok-Chun Yang

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  1. Chun-Gon Kim
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  2. Jong-Pyo Kang
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  3. Yue Huo
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  4. Mohan Chokkalingam
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  5. Yeon-Ju Kim
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  6. Dong-Hyun Kim
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Corresponding authors

Correspondence to Yeon-Ju Kim or Deok-Chun Yang.

Additional information

Communicated by Erko Stackebrandt.

The NCBI GenBank accession number for the 16S rRNA gene sequence of strain DCY114T is KY694399. DPD (digital protologue’ database) Taxon number: TA00398.

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Kim, CG., Kang, JP., Huo, Y. et al. Paenibacillus panacihumi sp. nov., a potential plant growth-promoting bacterium isolated from ginseng-cultivated soil. Arch Microbiol 200, 1049–1055 (2018). https://doi.org/10.1007/s00203-018-1517-z

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  • DOI: https://doi.org/10.1007/s00203-018-1517-z

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