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Luteibacter pinisoli sp. nov., a casein degrading bacterium isolated from rhizospheric soil of Pinus koraiensis

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Abstract

A yellow pigmented, Gram-staining negative, motile and rod-shaped novel bacterial strain, designated MAH-14T was isolated from rhizospheric soil and was characterized using a polyphasic approach. The isolated strain was aerobic, oxidase and catalase were positive, optimum growth temperature and pH were 28–30 °C and 6.5, respectively. The novel strain is able to hydrolyze casein, starch, esculin, gelatin, l-tyrosine, DNA, tween 80, tween 20, l-arginine and 4-nitrophenyl-BD-galactopyranoside. On the basis of 16S rRNA gene sequence analysis, strain MAH-14T belongs to the genus Luteibacter and is most closely related to Luteibacter yeojuensis R2A16-10T (98.5%), Luteibacter anthropi CCUG 25036T (98.4%) and Luteibacter rhizovicinus LJ96T (98.3%). In DNA–DNA hybridization experiments, the DNA relatedness between strain MAH-14T and its closest phylogenetic neighbor was below 45.0%. The predominant respiratory quinone and the DNA G + C content of the novel strain were ubiquinone-8 and 63.5 mol%, respectively. The novel strain MAH-14T is able to produce flexirubin-type pigments. The major cellular fatty acids were C15:0 iso, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and summed feature 9 (C17:1 iso ω9c and/or C16:0 10-methyl). The DNA–DNA hybridization results and results of the genotypic analysis in combination with chemotaxonomic and physiological data revealed that strain MAH-14T represented a novel species within the genus Luteibacter, for which the name Luteibacter pinisoli, is proposed. The type strain is MAH-14T (= KACC 19298T = CGMCC 1.16227T).

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Author information

Author notes

  1. Shahina Akter and Md. Amdadul Huq equally contributed to this work.

Authors and Affiliations

  1. Department of Horticultural Life Science, Hankyong National University, Anseong-si, Gyeonggi-do, 17579, Republic of Korea

    Shahina Akter

  2. Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong-si, Gyeonggi-do, 17546, Republic of Korea

    Md. Amdadul Huq

Authors
  1. Shahina Akter
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  2. Md. Amdadul Huq
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Corresponding author

Correspondence to Md. Amdadul Huq.

Additional information

Communicated by Erko Stackebrandt.

The NCBI GenBank accession number for the 16S rRNA gene sequence of strains MAH-14T is KY964279 and the digital protologue database (DPD) Taxon Number of strain MAH-14T is TA00441.

Electronic supplementary material

Below is the link to the electronic supplementary material.

203_2018_1515_MOESM1_ESM.pptx

Supplementary material 1 Supplementary Table 1. Negative properties of strain MAH-14T carried out by commercial test kits (API 20NE and API ZYM) (PPTX 45 KB)

203_2018_1515_MOESM2_ESM.pptx

Supplementary material 2 Supplementary Fig. S1. The maximum-likelihood (ML) tree based on 16S rRNA gene sequence analysis showing phylogenetic relationships of strain MAH-14T and members of the genus Luteibacter, values less than 50 % were not shown (PPTX 86 KB)

203_2018_1515_MOESM3_ESM.pptx

Supplementary material 3 Supplementary Fig. S2. Transmission electron micrograph of strain MAH-14T after negative staining with uranyl acetate, Bar, 1.0 μm (PPTX 455 KB)

203_2018_1515_MOESM4_ESM.pptx

Supplementary material 4 Supplementary Fig. S3. Casein degrading ability of strain MAH-14T. Clear zone around culture indicated that the strain MAH-14T is able to degrade casein (PPTX 649 KB)

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Akter, S., Huq, M.A. Luteibacter pinisoli sp. nov., a casein degrading bacterium isolated from rhizospheric soil of Pinus koraiensis. Arch Microbiol 200, 1017–1023 (2018). https://doi.org/10.1007/s00203-018-1515-1

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  • DOI: https://doi.org/10.1007/s00203-018-1515-1

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