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Halomarina rubra sp. nov., isolated from a marine solar saltern

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Abstract

Halophilic archaeal strain ZS-47-ST was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-ST was able to grow at 20–50 °C (optimum 37 °C), at 0.9–4.8 M NaCl (optimum 3.4 M), at 0.005–1.0 M MgCl2 (optimum 0.03 M), and at pH 5.5–9.5 (optimum pH 6.5–7.5). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell-lysis was 5% (w/v). The major polar lipids were C20C20 and C20C25 diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, glucosyl mannosyl glucosyl diether, and three unidentified glycolipids. The 16S rRNA gene and rpoB′ gene of strain ZS-47-ST were phylogenetically related to the corresponding genes of Halomarina oriensis JCM 16495T (98.57 and 92.94% similarities, respectively) and Halomarina salina CGMCC 1.12543T (97.96 and 93.65% similarities, respectively). The DNA G + C content of strain ZS-47-ST was 64.6 mol % (T m). The phenotypic, chemotaxonomic, and phylogenetic properties suggested that strain ZS-47-ST (=CGMCC 1.12563T = JCM 30037T) represents a new species of Halomarina, for which the name Halomarina rubra sp. nov. is proposed.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31370054) and the 11th “Six Talents Peak” Project of Jiangsu Province (No. 2014-SWYY-021).

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Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People’s Republic of China

    Yao Zhou, Yang Li, Zhen-Zhen Lü & Heng-Lin Cui

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  1. Yao Zhou
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  2. Yang Li
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  3. Zhen-Zhen Lü
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  4. Heng-Lin Cui
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Corresponding author

Correspondence to Heng-Lin Cui.

Additional information

Communicated by Erko Stackebrandt.

The Digital Protologue database Taxon Number for strain ZS-47-ST is TA00228. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and rpoB ′ gene sequences of strain ZS-47-ST are KC918830 and MF358965, respectively.

A phase-contrast micrograph of strain ZS-47-ST, thin-layer chromatograms of polar lipids extracted from strain ZS-47-ST, maximum-parsimony, and neighbour-joining phylogenetic tree reconstructions based on 16S rRNA gene and rpoB′ gene sequences showing the relationships between strain ZS-47-ST and related members within the class Halobacteria are available as supplementary materials.

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Zhou, Y., Li, Y., Lü, ZZ. et al. Halomarina rubra sp. nov., isolated from a marine solar saltern. Arch Microbiol 199, 1431–1435 (2017). https://doi.org/10.1007/s00203-017-1420-z

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  • DOI: https://doi.org/10.1007/s00203-017-1420-z

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