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Erythrobacter alti sp. nov., a marine alphaproteobacterium isolated from seawater

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Abstract

A polyphasic taxonomic study was performed on strain KMU-34T, which was isolated from seawater in the Republic of Korea. The bacterial cells were Gram-stain-negative, strictly aerobic, chemoheterotrophic, yellow-pigmented and rod-shaped. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain was affiliated with the family Erythrobacteraceae of the class Alphaproteobacteria and that it showed highest sequence similarity (98.6%) to Erythrobacter atlanticus s21-N3T. The DNA–DNA relatedness values between strains KMU-34T and E. atlanticus KCTC 42697T were 8.6 ± 1.2%. The DNA G + C content of strain KMU-34T was determined to be 60.4 mol%. Ubiquinone 10 (Q-10) was the sole respiratory quinone. The predominant cellular fatty acids were C18:1 ω7c (43.8%) and C16:1 ω7c (16.8%). Strain KMU-34T had phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, a sphingoglycolipid, an unidentified phospholipid and two unidentified lipids as polar lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel species of the genus Erythrobacter for which the name Erythrobacter alti sp. nov. is proposed. The type strain of E. alti sp. nov. is KMU-34T (= KCCM 90261T = NBRC 111903T).

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Acknowledgments

This research was supported by the Bisa Research Grant of Keimyung University in 2016.

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  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea

    Jaewoo Yoon

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  1. Jaewoo Yoon
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Correspondence to Jaewoo Yoon.

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Communicated by Erko Stackebrandt.

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203_2017_1384_MOESM1_ESM.pptx

Supplementary Fig. 1. Transmission electron micrograph of a negatively stained cell of strain KMU-34T. Bar, 500 nm (PPTX 1192 kb)

203_2017_1384_MOESM2_ESM.pptx

Supplementary Fig. 2. Thin-layer chromatograms showing the total polar lipid compositions of KMU-34T. Total polar lipids were detected by spraying the plate with molybdatophosphoric acid, molybdenun blue, α-naphthol and ninhydrin. PE: phosphatidylethanolamine, PG: phosphatidylglycerol, PC: phosphatidylcholine, SGL: sphingoglycolipid, UPL: unidentified phospholipid, UL: unidentified lipid (PPTX 401 kb)

Supplementary Fig. 3. Negative results from the commercial test kits (PPTX 43 kb)

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Yoon, J. Erythrobacter alti sp. nov., a marine alphaproteobacterium isolated from seawater. Arch Microbiol 199, 1133–1139 (2017). https://doi.org/10.1007/s00203-017-1384-z

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  • DOI: https://doi.org/10.1007/s00203-017-1384-z

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