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Co-effects of pyrene and nitrate on the activity and abundance of soil denitrifiers under anaerobic condition

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Abstract

It has previously been confirmed that polycyclic aromatic hydrocarbons (PAHs) could be degraded by soil microbes coupling with denitrification, but the relationships among soil denitrifiers, PAHs, and nitrate under obligate anaerobic condition are still unclear. Here, co-effects of pyrene and nitrate on the activity and abundance of soil denitrifiers were investigated through a 45-day incubation experiment. Two groups of soil treatments with (N30) and without (N0) nitrate (30 mg kg−1 dry soil) amendment were conducted, and each group contained three treatments with different pyrene concentrations (0, 30, and 60 mg kg−1 dry soil denoted as P0, P30, and P60, respectively). The pyrene content, abundances of denitrification concerning genes (narG, periplasmic nitrate reductase gene; nirS, cd 1-nitrite reductase gene; nirK, copper-containing nitrite reductase gene), and productions of N2O and CO2 were measured at day 3, 14, 28, and 45, and the bacterial community structures in four represented treatments (N0P0, N0P60, N30P0, and N30P60) were analyzed at day 45. The results indicated that the treatments with higher pyrene concentration had higher final pyrene removal rates than the treatments with lower pyrene concentration. Additionally, intensive emission of N2O was detected in all treatments only at day 3, but a continuous production of CO2 was measured in each treatment during the incubation. Nitrate amendment could enhance the activity of soil denitrifiers, and be helpful for soil microbes to sustain their activity. While pyrene seemed had no influence on the productions of N2O and CO2, and amendment with pyrene or nitrate both had no obvious effect on abundances of denitrification concerning genes. Furthermore, it was nitrate but not pyrene had an obvious influence on the community structure of soil bacteria. These results revealed that, under anaerobic condition, the activity and abundance of soil denitrifiers both were insensitive to pyrene, but nitrate could improve the activity of soil denitrfiers and induce the shifts in soil bacterial community structure.

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Acknowledgements

This work was supported by the Natural Science Foundation of China (41371477), and the Fundamental Research Funds for the Central Universities (XDJK2014B047).

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Authors and Affiliations

  1. College of Resources and Environment, Southwest University, Chongqing, 400716, China

    Zhi-Feng Zhou, Yan-Hong Yao, Ming-Xia Wang & Xiao-Hu Zuo

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  1. Zhi-Feng Zhou
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  2. Yan-Hong Yao
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Correspondence to Zhi-Feng Zhou.

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Communicated by Erko Stackebrandt.

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Zhou, ZF., Yao, YH., Wang, MX. et al. Co-effects of pyrene and nitrate on the activity and abundance of soil denitrifiers under anaerobic condition. Arch Microbiol 199, 1091–1101 (2017). https://doi.org/10.1007/s00203-017-1380-3

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  • DOI: https://doi.org/10.1007/s00203-017-1380-3

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