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Frankia discariae sp. nov.: an infective and effective microsymbiont isolated from the root nodule of Discaria trinervis

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Abstract

Strain BCU110501T was the first isolate reported to fulfill Koch’s postulates by inducing effective nodules on its host plant of origin Discaria trinervis (Rhalmnaceae). Based on 16S rRNA gene sequence similarities, the strain was found to be most closely related to the type strain of Frankia elaeagni DSM 46783T (98.6%) followed by F. alni DSM 45986T (98.2%), F. casuarinae DSM 45818T (97.8%) and F. inefficacies DSM 45817T (97.8%). Digital DNA:DNA hybridizations (dDDH) between strain BCU110501Tand the type strains of other Frankia species were clearly below the cutoff point of 70%. The G+C content of DNA is 72.36%. The cell wall of strain BCU110501T contained meso-diaminopimelic acid and the cell sugars were galactose, glucose, mannose, xylose and ribose. Polar lipids were phosphatidylinositol (PI), diphosphatidylglycerol (DPG), glycophospholipid (GPL1−3), phosphatidylglycerol (PG) and an unknown lipid (L). The major fatty acids of strain BCU110501T consisted of iso-C16:0, C17:1 w8c and C16:0. Major menaquinones were MK9 (H4), MK9 (H6) and MK9 (H2). Based on these analyses, strain BCU110501T (=DSM 46785T=CECT 9042T) should be classified as the type strain of a novel Frankia species, for which the name Frankia discariae sp. nov. is proposed.

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Acknowledgements

This work was supported by the Laboratoire Microorganismes & Biomolécules Actives, Université Tunis El-Manar, Tunisia (Grant LR03ES03). We are grateful to Marlen Jando and Gabriele Pötter for help with the chemotaxonomic analyses and to Brian J. Tindall (all at DSMZ) for helpful advice.

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Authors and Affiliations

  1. Laboratoire Microorganismes et Biomolécules Actives, Université Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia

    Imen Nouioui, Faten Ghodhbane-Gtari & Maher Gtari

  2. School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK

    Imen Nouioui, Maria del Carmen Montero-Calasanz & Hans-Peter Klenk

  3. HZI-Helmholtz Centre for Infection Research, Inhoffenstraße7, 38124, Brunswick, Germany

    Manfred Rohde

  4. Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, 289 Rudman Hall, 46 college Road, Durham, NH, 03824-2617, USA

    Louis S. Tisa

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  1. Imen Nouioui
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Correspondence to Maher Gtari.

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Communicated by Erko Stackebrandt.

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Nouioui, I., del Carmen Montero-Calasanz, M., Ghodhbane-Gtari, F. et al. Frankia discariae sp. nov.: an infective and effective microsymbiont isolated from the root nodule of Discaria trinervis . Arch Microbiol 199, 641–647 (2017). https://doi.org/10.1007/s00203-017-1337-6

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