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Low copy number and high 4977 deletion of mitochondrial DNA in uterosacral ligaments are associated with pelvic organ prolapse progression

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Abstract

Introduction and hypothesis

The pathophysiology of pelvic organ prolapse (POP) is related to aging in the pelvic organ support, and mitochondrial dysfunction is one of the major contributors to aging. Therefore, the objective of this study was to investigate the correlation between alternations of mitochondrial DNA and progression of POP.

Methods

Polymerase chain reaction (PCR) was applied in the present study. Uterosacral ligaments (UL) were obtained from 45 POP patients and 38 myoma patients without POP. Chi-square test, Student’s t-test, Mann–Whitney U test, and Spearman correlation analysis were applied in the comparison between POP and non-POP patients.

Results

The results revealed that significant depletion of mitochondrial DNA (mtDNA) and an increase in the incidence of 4977 deletion of mtDNA (mtDNA4977) in the UL tissue of POP patients.

Conclusions

The alternations of mtDNA may play an important role in the molecular pathogenesis and process of POP formation.

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Abbreviations

POP:

pelvic organ prolapse

QPCR:

quantitative polymerase chain reaction

UL:

uterosacral ligaments

mtDNA:

mitochondrial DNA

mtDNA4977:

4977 deletion of mitochondrial DNA

BMI:

body-mass index

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Acknowledgments

The authors wish to thank Chih-Cheng Huang, MD, Man-Chi Lo, MD, and the Changhua Christian Hospital, Changhua, Taiwan, for their cooperation and assistance with this study.

Conflicts of interest

None.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua, Taiwan

    Mou-Jong Sun

  2. General Education Center, Chienkuo Technology University, Changhua, Taiwan

    Mou-Jong Sun

  3. Department of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan

    Yau-Huei Wei

  4. Vascular and Genomic Center, Changhua Christian Hospital, 135 Nanhsiao Street, Changhua, 500, Taiwan

    Wen-Ling Cheng, Chen-Ling Kuo, Hui-Mei Lin & Chin-San Liu

  5. Department of Biochemistry, University of Washington, Seattle, WA, USA

    Samuel Sun

  6. Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua, Taiwan

    Horng-Der Tsai

  7. Department of Neurology, Changhua Christian Hospital, Changhua, Taiwan

    Chin-San Liu

  8. Graduate Institute of Integration Chinese and Western Medicine, China Medical University, Taichung, Taiwan

    Chin-San Liu

Authors
  1. Mou-Jong Sun
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  2. Wen-Ling Cheng
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  3. Yau-Huei Wei
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  4. Chen-Ling Kuo
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  5. Samuel Sun
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  6. Horng-Der Tsai
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  7. Hui-Mei Lin
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  8. Chin-San Liu
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Corresponding authors

Correspondence to Hui-Mei Lin or Chin-San Liu.

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Sun, MJ., Cheng, WL., Wei, YH. et al. Low copy number and high 4977 deletion of mitochondrial DNA in uterosacral ligaments are associated with pelvic organ prolapse progression. Int Urogynecol J 20, 867–872 (2009). https://doi.org/10.1007/s00192-009-0871-4

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  • DOI: https://doi.org/10.1007/s00192-009-0871-4

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