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Induction of specific stress response increases resistance of rat liver allografts to cold ischemia and reperfusion injury

  • Original Article
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Transplant International

Abstract

Heme oxygenase-1 (HO-1) has been shown to increase cellular resistance against oxidative injury, but the functional significance of this is currently obscure. We investigated the protective role of HO-1, induced by tin-protoporphyrin IX (SnPP), in attenuating liver transplantation injury. Lewis rats were intraperitoneally treated with saline as control, 50 µmol/kg of SnPP, or 2 mg/kg of cycloheximide (CHX) before SnPP injection. Gene expression of HO-1 was induced after either treatment with SnPP- or CHX + SnPP instead of saline, whereas HO-1 protein synthesis was enhanced in Kupffer-like dendritic cells of the SnPP-treated group. Following reperfusion of liver grafts preserved for 30 h, there were fewer intercellular adhesion molecule-1-positive cells in SnPP-treated livers, significantly reduced numbers of dead cells, and enhanced graft viability. The present data suggest that increased synthesis of HO-1 protein by SnPP pre-conditioning is linked to the improved liver graft viability through inhibition of inflammatory adhesion molecules.

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Acknowledgements

The authors are very grateful to Drs. Makoto Suematsu and Nobuya Makino of Keio University for their excellent suggestions. We also thank Dr. Andrew H. Lichtman, Department of Pathology, Brigham and Women's Hospital, Boston, Mass., USA for correcting this manuscript.

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Authors and Affiliations

  1. Research Institute for Artificial Organs, Sapporo Hokuyu Hospital, Higashi-Sapporo 6-6, Shiroishi-ku, 003-0006, Sapporo, Japan

    Yasushi Uchida, Tohru Tamaki, Mitsuko Tanaka, Takashi Kaizu, Sei-ichiro Tsuchihashi & Akio Kawamura

  2. Department of Surgery, Kitasato University School of Medicine, Kanagawa, Japan

    Yasushi Uchida, Takashi Kaizu, Tsuyoshi Takahashi & Akira Kakita

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  1. Yasushi Uchida
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  2. Tohru Tamaki
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  3. Mitsuko Tanaka
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  6. Tsuyoshi Takahashi
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Correspondence to Tohru Tamaki.

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Uchida, Y., Tamaki, T., Tanaka, M. et al. Induction of specific stress response increases resistance of rat liver allografts to cold ischemia and reperfusion injury. Transpl Int 16, 396–404 (2003). https://doi.org/10.1007/s00147-003-0550-1

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  • DOI: https://doi.org/10.1007/s00147-003-0550-1

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