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Surface triggering receptor expressed on myeloid cells 1 expression patterns in septic shock

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Abstract

Objective

To analyze the pattern of cell-surface expression of the triggering receptor expressed on myeloid cells (TREM) 1 during septic shock.

Design and setting

Prospective clinical study in an adult 16-bed medical ICU.

Patients and methods

25 septic shock patients, 15 patients with shock of noninfectious origin and 7 healthy volunteers. Arterial blood was drawn within 12 h of admission and subjected to flow cytometry analysis after staining with anti-TREM-1 and anti-CD14 antibodies. Repeated sampling was performed on days 2, 3, 5, 7, and 14 in septic shock patients.

Results

Monocytic TREM-1 expression was significantly higher in septic shock patients (mean fluorescence intensity 2.3±0.2) than in nonseptic patients (1.0±0.1), and healthy volunteers (1.0±0.1). There was no difference in monocytic TREM-1 expression between nonseptic patients and healthy volunteers or between any of the three groups with respect to TREM-1 expression on neutrophils. The time course of TREM-1 expression on monocytes diverged significantly by day 3 between survivors and ns.

Conclusions

The specificity of TREM-1 regulation by infection is highlighted. Moreover, surface TREM-1 expression on monocytes may prove useful in allowing the follow-up of septic patients during the course of the disease

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Acknowledgements

This work was supported in part by a grant from the Programme Hospitalier de Recherche Clinique (PHRC).

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Authors and Affiliations

  1. Réanimation Médicale, Hôpital Central, 54000, Nancy, France

    Sébastien Gibot, Pierre-Edouard Bollaert & Bruno Lévy

  2. Laboratoire de Physiologie Expérimentale, Hôpital Central, 54000, Nancy, France

    Sébastien Gibot & Bruno Lévy

  3. Laboratoire d’Immunologie, Hôpital Central, 54000, Nancy, France

    Pol-Edern Le Renard, Marie-Nathalie Kolopp-Sarda, Marie-Christine Béné & Gilbert C. Faure

Authors
  1. Sébastien Gibot
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  2. Pol-Edern Le Renard
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  3. Pierre-Edouard Bollaert
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  4. Marie-Nathalie Kolopp-Sarda
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  5. Marie-Christine Béné
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  6. Gilbert C. Faure
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  7. Bruno Lévy
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Corresponding author

Correspondence to Sébastien Gibot.

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Gibot, S., Le Renard, PE., Bollaert, PE. et al. Surface triggering receptor expressed on myeloid cells 1 expression patterns in septic shock. Intensive Care Med 31, 594–597 (2005). https://doi.org/10.1007/s00134-005-2572-x

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  • DOI: https://doi.org/10.1007/s00134-005-2572-x

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