Skip to main content
SpringerLink
Log in

Resistance to powdery mildew in Spanish barley landraces is controlled by different sets of quantitative trait loci

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Twenty-two landrace-derived inbred lines from the Spanish Barley Core Collection (SBCC) were found to display high levels of resistance to a panel of 27 isolates of the fungus Blumeria graminis that exhibit a wide variety of virulences. Among these lines, SBCC145 showed high overall resistance and a distinctive spectrum of resistance compared with the other lines. Against this background, the main goal of the present work was to investigate the genetic basis underlying such resistance using a doubled haploid population derived from a cross between SBCC145 and the elite spring cultivar Beatrix. The population was genotyped with the 1,536-SNP Illumina GoldenGate Oligonucleotide Pool Assay (Barley OPA-1 or BOPA1 for short), whereas phenotypic analysis was performed using two B. graminis isolates. A major quantitative trait locus (QTL) for resistance to both isolates was identified on the long arm of chromosome 6H (6HL) and accounted for ca. 60% of the phenotypic variance. Depending on the B. graminis isolate tested, three other minor QTLs were detected on chromosomes 2H and 7H, which explained less than 5% of the phenotypic variation each. In all cases, the alleles for resistance derived from the Spanish parent SBCC145. The position, the magnitude of the effect observed and the proportion of phenotypic variation accounted for by the QTL on 6HL suggest this is a newly identified locus for broad-based resistance to powdery mildew.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Aghnoum R, Niks RE (2011) Transgressive segregation for very low and high levels of basal resistance to powdery mildew in barley. J Plant Physiol 168:45–50

    Article  PubMed  CAS  Google Scholar 

  • Aghnoum R, Marcel TC, Johrde A, Pecchioni N, Schweizer P, Niks RE (2010) Basal host resistance of barley to powdery mildew: connecting quantitative trait loci and candidate genes. Mol Plant Microbe Interact 23:91–102

    Article  PubMed  CAS  Google Scholar 

  • Ayliffe MA, Collins NC, Ellis JG, Pryor A (2000) The maize rp1 rust resistance gene identifies homologues in barley that have been subjected to diversifying selection. Theor Appl Genet 100:1144–1154

    Article  CAS  Google Scholar 

  • Backes G, Graner B, Foroughi-Wehr G, Fischbeck G, Wenzel G, Jahoor A (1995) Localization of quantitative trait loci (QTL) for agronomic important characters by the use of a RFLP map in barley (Hordeum vulgare L.). Theor Appl Genet 90:294–302

    Article  CAS  Google Scholar 

  • Backes G, Madsen LH, Jaiser H, Stougaard J, Herz M, Mohler V, Jahoor A (2003) Localization of genes for resistance against Blumeria graminis f.sp. hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley (Hordeum vulgare ssp. spontaneum) line. Theor Appl Genet 106:353–362

    PubMed  CAS  Google Scholar 

  • Brown JKM, Jørgensen JH (1991) A catalogue of mildew resistance genes in European barley varieties. In: Jørgensen JH (ed) Integrated control of cereal mildews: virulence patterns and their change. Risø National Laboratory, Roskilde, Denmark, pp 263–286

    Google Scholar 

  • Brueggeman R, Rostoks N, Kurdna D, Kilian A, Han F, Chen J, Druka A, Steffenson BJ, Kleinhofs A (2002) The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases. Proc Natl Acad Sci USA 99:9328–9333

    Article  PubMed  CAS  Google Scholar 

  • Bulgarelli D, Collins NC, Tacconi G, Dellaglio E, Brueggeman R, Kleinhofs A, Stanca AM, Valè G (2004) High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley. Theor Appl Genet 108:1401–1408

    Article  PubMed  CAS  Google Scholar 

  • Bundessortenamt (2009) Ergänzende feststellungen zur anfälligkeit für krankheiten und schädlinge. In: Beschreibende Sortenliste für Getreide, mais, ölfrüchte, leguminosen, hackfrüchte, pp 44–49

  • Close TJ, Bhat PR, Lonardi S, Wu Y, Rostoks N, Ramsay L, Druka A, Stein N, Svensson J, Wanamaker S, Bozdag S, Roose M, Moscou M, Chao S, Varshney R, Szucs P, Sato K, Hayes P, Matthews D, Kleinhofs A, Muehlbauer G, DeYoung J, Marshall D, Madishetty K, Fenton R, Condamine P, Graner A, Waugh R (2009) Development and implementation of high-throughput SNP genotyping in barley. BMC Genomics 10:582

    Article  PubMed  Google Scholar 

  • Cockram J, White J, Zuluaga D, Comadran J, Macaulay M, Liu Z, Kearsey M, Werner P, Harrap D, Tapsell C, Liu H, Stein N, Schulte D, Steurnagel B, Marshall DF, Thomas WTB, Ramsay L, Mackay I, Balding DJ, the AGOUEB Consortium, Waugh R, O’Sullivan DM (2010) Genome-wide association mapping of morphological traits to candidate gene resolution in the un-sequenced barley genome. Proc Natl Acad Sci USA 107:21611–21616

    Article  PubMed  CAS  Google Scholar 

  • Comadran J, Thomas WTB, van Eeuwijk F, Ceccarelli S, Grando S, Stanca AM, Pecchioni N, Akar T, Al-Yassin A, Benbelkacem A, Ouabbou H, Bort J, Romagosa I, Hackett CA, Russell JR (2009) Patterns of genetic diversity and linkage disequilibrium in a highly structured Hordeum vulgare association-mapping population for the Mediterranean basin. Theor Appl Genet 119:175–187

    Article  PubMed  CAS  Google Scholar 

  • Czembor JH (1999) Resistance to powdery mildew in barley landraces from Tunisia. Plant Breed Seed Sci 43:49–65

    Google Scholar 

  • Czembor JH (2000) Resistance to powdery mildew in populations of barley landraces from Morocco. Genet Res Crop Evol 47:439–450

    Article  Google Scholar 

  • Drader T, Johnson K, Brueggeman R, Kudrna D, Kleinhofs A (2009) Genetic and physical mapping of a high recombination region on chromosome 7H(1) in barley. Theor Appl Genet 118:811–820

    Article  PubMed  CAS  Google Scholar 

  • Dreiseitl A, Bockelman HE (2003) Sources of powdery mildew resistance in a wild barley collection. Genet Resour Crop Evol 50:345–350

    Article  Google Scholar 

  • Falak I, Falk DE, Tinker NA, Mather DE (1999) Resistance to powdery mildew in a doubled-haploid barley population and its association with marker loci. Euphytica 107:185–192

    Article  CAS  Google Scholar 

  • Fischbeck G (1992) Barley cultivar development in Europe. Success in the past and possible changes in the future. In: Munk L (ed) Proceedings of 6th international barley genetics, vol II. Munksgaard Int Publ Ltd. Copenhagen, pp 887–901

  • Fischbeck G (2003) Diversification through breeding. In: von Bothmer R, van Hintum T, Knuepffer H, Sato K (eds) Diversity in barley. Elsevier, Amsterdam, pp 29–52

    Chapter  Google Scholar 

  • Flor HH (1971) Current status of the gene-for-gene concept. Ann Rev Phytopathol 9:275–296

    Article  Google Scholar 

  • Friedt W, Ordon F (2007) Molecular markers for gene pyramiding and disease resistance breeding in barley. In: Varshney RV, Tuberosa R (eds) Genomics-assisted crop improvement, vol 2: genomics application in crops. Springer, Netherlands, pp 81–101

    Chapter  Google Scholar 

  • Hajjar R, Hodgkin T (2007) The use of wild relatives in crop improvement: a survey of developments over the last 20 years. Euphytica 156:1–13

    Article  Google Scholar 

  • Heun M (1992) Mapping quantitative powdery mildew resistance of barley using a restriction fragment length polymorphism map. Genome 35:1019–1025

    Article  CAS  Google Scholar 

  • Huxley A (2005) Green inheritance. University of California Press, Berkeley and Los Angeles, California

    Google Scholar 

  • Igartua E, Gracia MP, Lasa JM, Medina B, Molina-Cano JL, Montoya JL, Romagosa I (1998) The Spanish barley core collection. Genet Resour Crop Evol 45:475–481

    Article  Google Scholar 

  • Jahoor A (1986) Mehltauresistenz israelischer Wildgersten—Resistenzspektrum, Vererbung und Lokalisierung. Dissertation, Technische Universität München, Freising-Weihenstephan

  • Jahoor A, Fischbeck G (1993) Identification of new genes for mildew resistance of barley at the Mla locus in lines derived from Hordeum spontaneum. Plant Breed 110:116–122

    Article  Google Scholar 

  • Jansen R, Stam P (1994) High resolution of quantitative traits into multiple loci via interval mapping. Genetics 136:1447–1455

    PubMed  CAS  Google Scholar 

  • Johrde A, Schweizer P (2008) A class III peroxidase specifically expressed in pathogen-attacked barley epidermis contributes to basal resistance. Mol Plant Pathol 9:687–696

    Article  PubMed  CAS  Google Scholar 

  • Jørgensen JH (1994) Genetics of powdery mildew resistance in barley. Critic Rev Plant Sci 13:97–119

    Article  Google Scholar 

  • Kølster P, Munk L, Stølen O, Løhde J (1986) Near-isogenic barley lines with genes for resistance to powdery mildew. Crop Sci 26:903–907

    Article  Google Scholar 

  • Lasa JM (2008) Spanish Barley Core Collection. Monografías INIA: Serie Agrícola, n. 25. Instituto Nacional de Tecnología Agraria y Alimentaria, Madrid

    Google Scholar 

  • Li HB, Zhou MX (2011) Quantitative trait loci controlling barley powdery mildew and scald resistances in two different barley doubled haploid populations. Mol Breeding 27:479–490

    Article  Google Scholar 

  • Marcel TC, Varshney RK, Barbieri M, Jafary H, de Kock MJD, Graner A, Niks RE (2007) A high-density consensus map of barley to compare the distribution of QTL for partial resistance to Puccinia hordei and of defence gene homologues. Theor Appl Genet 114:487–500

    Article  PubMed  CAS  Google Scholar 

  • Ordon F (2009) Coordinator's report: disease and pest resistance genes. In: Udda Lundqvist (ed) Barley genetics newsletter 39, pp 58–69

  • Řepková J, Dreiseitl A, Lízal P, Kyjovská Z, Teturová K, Psotková R, Jahoor A (2006) Identification of resistance genes against powdery mildew in four accessions of Hordeum vulgare ssp spontaneum. Euphytica 151:23–30

    Article  Google Scholar 

  • Roy JK, Smith KP, Muehlbauer GJ, Chao S, Close TJ, Steffenson BJ (2010) Association mapping of spot blotch resistance in wild barley. Mol Breeding 26:243–256

    Article  Google Scholar 

  • Saghai Maroof MA, Blyashev RB, Zhang Q (1994) Molecular marker analysis of powdery mildew resistance in barley. Theor Appl Genet 88:733–740

    Article  Google Scholar 

  • Sato K, Takeda K (2009) An application of high-throughput SNP genotyping for barley genome mapping and characterization of recombinant chromosome substitution lines. Theor Appl Genet 119:613–619

    Article  PubMed  CAS  Google Scholar 

  • Schönfeld M, Ragni A, Fischbeck G, Jahoor A (1996) RFLP mapping of three new loci for resistance genes to powdery mildew (Erysiphe graminis f. sp. hordei) in barley. Theor Appl Genet 93:48–56

    Article  Google Scholar 

  • Shtaya MJY, Marcel TC, Sillero JC, Niks RE, Rubiales D (2006) Identification of QTLs for powdery mildew and scald resistance in barley. Euphytica 151:421–429

    Article  Google Scholar 

  • Shtaya MJY, Sillero JC, Flath K, Pickering R, Rubiales D (2007) The resistance of leaf rust and powdery mildew of recombinant lines of barley (Hordeum vulgare L.) derived from H. vulgare × H. bulbosum crosses. Plant Breed 126:259–267

    Article  CAS  Google Scholar 

  • Silvar C, Casas AM, Kopahnke D, Habekuß A, Schweizer G, Gracia MP, Lasa JM, Ciudad FJ, Molina-Cano JL, Igartua E, Ordon F (2010a) Screening the Spanish barley core collection for disease resistance. Plant Breed 129:45–52

    Article  Google Scholar 

  • Silvar C, Dhif H, Igartua E, Kopahnke D, Gracia MP, Lasa JM, Ordon F, Casas AM (2010b) Identification of quantitative trait loci for resistance to powdery mildew in a Spanish barley landrace. Mol Breed 25:581–592

    Article  CAS  Google Scholar 

  • Silvar C, Flath K, Kopahnke D, Gracia MP, Lasa JM, Casas AM, Igartua E, Ordon F (2011a) Analysis of powdery mildew resistance in the Spanish barley core collection. Plant Breed 130:195–202

    Article  Google Scholar 

  • Silvar C, Perovic D, Casas AM, Igartua E, Ordon F (2011b) Development of a cost-effective pyrosequencing approach for SNP genotyping in barley. Plant Breed 130:394–397

    Google Scholar 

  • Szűcs P, Blake VC, Bhat PR, Chao S, Close TJ, Cuesta-Marcos A, Muehlbauer GJ, Ramsay L, Waugh R, Hayes PM (2009) An integrated resource for barley linkage map and malting quality QTL alignment. Plant Genome 2:134–140

    Article  Google Scholar 

  • Trujillo M, Troeger M, Niks RE, Kogel KH, Hückelhoven R (2004) Mechanistic and genetic overlap of barley host and non-host resistance to Blumeria graminis. Mol Plant Pathol 5:389–396

    Article  PubMed  CAS  Google Scholar 

  • van Ooijen JW (2004) MapQTL® 5, Software for the mapping of quantitative trait loci in experimental populations. Kyazma BV, Wageningen, Netherlands

    Google Scholar 

  • van Ooijen JW (2006) JoinMap 4, software for the calculation of genetics linkage maps in experimental populations. Kyazma, BV, Wageningen, Netherlands

    Google Scholar 

  • von Korff M, Wang H, Léon J, Pillen K (2005) AB-QTL analysis in spring barley. I. Detection of resistance genes against powdery mildew, leaf rust and scald introgressed from wild barley. Theor Appl Genet 111:583–590

    Article  Google Scholar 

  • Wang Z, Taramino G, Yang D, Liu G, Tingey SV, Miao GH, Wang GL (2001) Rice ESTs with disease-resistance gene- or defense response gene-like sequences mapped to regions containing major resistance genes or QTL. Mol Genet Genomics 265:302–310

    Article  PubMed  CAS  Google Scholar 

  • Williams KJ (2003) The molecular genetics of disease resistance in barley. Aust J Agric Res 54:1065–1079

    Article  CAS  Google Scholar 

  • Xu S (2008) Quantitative trait locus mapping can benefit from segregation distortion. Genetics 180:2201–2208

    Article  PubMed  Google Scholar 

  • Yun SJ, Gyenis L, Hayes PM, Matus I, Smith KP, Steffenson BJ, Muehlbauer GJ (2005) Quantitative trait loci for multiple disease resistance in wild barley. Crop Sci 45:2563–2572

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was funded by the Spanish Ministry of Science and Innovation (projects GEN2006-28560-E, AGL2007-63625 and Plant-KBBE ExpResBar, EUI2009-04075) and co-funded by the European Regional Development Fund. CS holds an I3P postdoctoral contract from CSIC. CS was supported by mobility fellowships from the Deutsche Forschungsgemeinschaft (DFG), CSIC, Fundación Caja Inmaculada and COST Action FA0604 (Tritigen).

Author information

Author notes

  1. C. Silvar

    Present address: Department of Ecology, Plant and Animal Biology, University of Coruña, Campus da Zapateira s/n, 15071, A Coruña, Spain

  2. L. J. Ponce-Molina

    Present address: National Autonomous Institute for Agricultural Research (INIAP), Santa Catalina Experimental Station, Panamericana Sur Km1, Quito, Ecuador

Authors and Affiliations

  1. Department of Genetics and Plant Production, Aula Dei Experimental Station, EEAD-CSIC, Avda Montañana 1005, 50059, Zaragoza, Spain

    C. Silvar, A. M. Casas, E. Igartua, L. J. Ponce-Molina & M. P. Gracia

  2. Institute for Crop Science and Plant Breeding, Bavarian State Research Centre for Agriculture, Am Gereuth 6, 85354, Freising-Weihenstephan, Germany

    G. Schweizer & M. Herz

  3. Institute for Plant Protection in Field Crops and Grassland, Julius Kühn-Institute, 14532, Kleinmachnow, Germany

    K. Flath

  4. The James Hutton Institute, Invergowrie, DD2 5NE, Dundee, Scotland, UK

    R. Waugh

  5. Institute for Resistance Research and Stress Tolerance, Julius Kühn Institute (JKI), Federal Research Institute for Cultivated Plants, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    D. Kopahnke & F. Ordon

Authors
  1. C. Silvar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. M. Casas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Igartua
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. J. Ponce-Molina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. P. Gracia
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. Schweizer
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. Herz
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. K. Flath
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. R. Waugh
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. D. Kopahnke
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. F. Ordon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. Igartua.

Additional information

Communicated by P. Hayes.

Electronic supplementary material

Below is the link to the electronic supplementary material.

SBCC145 × Beatrix BOPA1 map

122_2011_1644_MOESM2_ESM.doc

Segregation distortion for BOPA1 SNPs in the SBCC145 × Beatrix population. Estimated positions of PpdH1, EBmac640 (closely linked to Eam6), vrs1, denso, VrnH2, VrnH1 and VrnH3 are depicted. Chi-square values are plotted in the lower part of the graph

Rights and permissions

Reprints and permissions

About this article

Cite this article

Silvar, C., Casas, A.M., Igartua, E. et al. Resistance to powdery mildew in Spanish barley landraces is controlled by different sets of quantitative trait loci. Theor Appl Genet 123, 1019–1028 (2011). https://doi.org/10.1007/s00122-011-1644-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-011-1644-2

Keywords

Search

Navigation