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Identification of two novel genes for blackleg resistance in Brassica napus

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Abstract

Blackleg, caused by Leptosphaeria maculans, is a major disease of Brassica napus. Two populations of B. napus DH lines, DHP95 and DHP96, with resistance introgressed from B. rapa subsp. sylvestris, were genetically mapped for resistance to blackleg disease with restriction fragment length polymorphism markers. Examination of the DHP95 population indicated that a locus on linkage group N2, named LepR1, was associated with blackleg resistance. In the DHP96 population, a second locus on linkage group N10, designated LepR2, was associated with resistance. We developed BC1 and F2 populations, to study the inheritance of resistance controlled by the genes. Genetic analysis indicated that LepR1 was a dominant nuclear allele, while LepR2 was an incompletely dominant nuclear resistance allele. LepR1 and LepR2 cotyledon resistance was further evaluated by testing 30 isolates from Canada, Australia, Europe, and Mexico. The isolates were from B. napus, B. juncea, and B. oleracea and represented different pathogenicity groups of L. maculans. Results indicated that LepR1 generally conferred a higher level of cotyledon resistance than LepR2. Both genes exhibited race-specific interactions with pathogen isolates; virulence on LepR1 was observed with one isolate, pl87-41, and two isolates, Lifolle 5, and Lifolle 6, were virulent on LepR2. LepR1 prevented hyphal penetration, while LepR2 reduced hyphal growth and inhibited sporulation. Callose deposition was associated with resistance for both loci.

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Acknowledgements

We would like to thank Mr. Greg Buzza, of Advanta Canada, for providing lines of two populations of doubled haploid lines of B. napus that had been evaluated for stem resistance in field nurseries in Australia and for pedigrees of the materials. We thank Drs. I.A.P. Parkin and R. Rusholme for help with RFLP analysis, Ms. S. Rosloski and Mr. Rashid Nur for assistance with disease evaluation. Special thanks go to Dr. M. Keri for providing many isolates of L. maculans for this study. We acknowledge Dr. G. Séguin-Swartz for providing light microscopy facilities and help with cytological research. We also wish to thank Mr. R.E. Underwood for assistance in preparation of the figures. This work was funded by a grant from Saskatchewan Agriculture and Food, Agriculture Development Fund.

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  1. Saskatoon Research Center, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, S7N 0X2, Canada

    F. Yu, D. J. Lydiate & S. R. Rimmer

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  1. F. Yu
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  2. D. J. Lydiate
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  3. S. R. Rimmer
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Correspondence to S. R. Rimmer.

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Yu, F., Lydiate, D.J. & Rimmer, S.R. Identification of two novel genes for blackleg resistance in Brassica napus . Theor Appl Genet 110, 969–979 (2005). https://doi.org/10.1007/s00122-004-1919-y

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