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Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion

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An Author Correction to this article was published on 22 March 2018

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Abstract

Secondary damage following spinal cord injury leads to non-reversible lesions and hampering of the reparative process. The local production of pro-inflammatory cytokines such as TNF-α can exacerbate these events. Oligodendrocyte death also occurs, followed by progressive demyelination leading to significant tissue degeneration. Dental stem cells from human apical papilla (SCAP) can be easily obtained at the removal of an adult immature tooth. This offers a minimally invasive approach to re-use this tissue as a source of stem cells, as compared to biopsying neural tissue from a patient with a spinal cord injury. We assessed the potential of SCAP to exert neuroprotective effects by investigating two possible modes of action: modulation of neuro-inflammation and oligodendrocyte progenitor cell (OPC) differentiation. SCAP were co-cultured with LPS-activated microglia, LPS-activated rat spinal cord organotypic sections (SCOS), and LPS-activated co-cultures of SCOS and spinal cord adult OPC. We showed for the first time that SCAP can induce a reduction of TNF-α expression and secretion in inflamed spinal cord tissues and can stimulate OPC differentiation via activin-A secretion. This work underlines the potential therapeutic benefits of SCAP for spinal cord injury repair.

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  • 22 March 2018

    In the original publication, sixth author’s surname was incorrectly published as “Llyod” instead of “Lloyd”. The correct name should read as “Amy Lloyd”.

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Acknowledgements

Anne des Rieux is a Research Associate, Mireille Alhouayek is a Post-doctoral Researcher and Pauline Bottemanne is a FRIA Doctoral Researcher at the FRS-FNRS (Fonds de la Recherche Scientifique). The authors acknowledge Prof. O. Feron (UCL) for the access to hypoxia incubator and Daniel Soong (EdU) for his help with MBP quantification as well as Loïc Germain (UCL) for his support in the development of the tri-cultures. We are also grateful to Université Catholique de Louvain (FSR) and International Foundation for Research in Paraplegia (IRP) for the financial support. The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.

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Author notes

  1. Giulio G. Muccioli and Anne des Rieux equally contributed to the work.

Authors and Affiliations

  1. Louvain Drug Research Institute (LDRI), Advanced Drug Delivery and Biomaterials (ADDB), Université Catholique de Louvain, Avenue E. Mounier 73, B1 73.12, 1200, Brussels, Belgium

    Pauline De Berdt, John Bianco & Anne des Rieux

  2. Louvain Drug Research Institute, Bioanalysis and Pharmacology of Bioactive Lipids Research Group (BPBL), Université Catholique de Louvain, Avenue E. Mounier 73, B1 72.01, 1200, Brussels, Belgium

    Pauline Bottemanne, Mireille Alhouayek & Giulio G. Muccioli

  3. Department of Endodontics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA

    Anibal Diogenes

  4. MRC Center for Reproductive Health, The Queen’s Medical Research Institute, The University of Edinburgh, Edinburgh, UK

    Amy Llyod & Véronique Miron

  5. Institute of Neuropathology, Uniklinik RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany

    Jose Gerardo-Nava & Gary A. Brook

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Results obtained for LPS-treated samples are presented in this manuscript, while results for non-LPS-treated samples are in supplementary data.

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De Berdt, P., Bottemanne, P., Bianco, J. et al. Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion. Cell. Mol. Life Sci. 75, 2843–2856 (2018). https://doi.org/10.1007/s00018-018-2764-5

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