Abstract
The changes in the number of CD8+ T lymphocytes were studied before (0 day) and then 30 days after the autologous hematopoietic stem cell transplantations (AHSCT) in 14 therapy refractory patients with autoimmune diseases. The years of survival and the clinical states were also evaluated. The number of CD8+ T cells was determined by an hematologic automat and by flow cytometry. Longer than 5-year survival times were found in 6 cases, whereas there was no progression (improvement) in 2 cases, and 4 patients were lost. The increase in the number of CD8+ cytotoxic T cells was gradual in the first 2 months and reached the significantly highest values among all subtypes of lymphocytes. It was of a special interest that in all the 4 patients who died, the numbers of CD8+ T cells were less than 150/μl on the 30th day after AHSCT, whereas all the 10 patients with a higher cell number survived. These results suggest that the early monitoring of the number (not only the ratio) of regenerating CD8+ T cells in the peripheral blood can be a useful and quantitative laboratory measurement after AHSCT, and it has a significant relation also to the survival times of transplanted patients.
References
Alchi B, Jayne D, Labopin M et al (2013) Autologous haematopoietic stem cell transplantation for systemic lupus erythematosus: data from the European group for blood and marrow transplantation registry. Lupus 22:245–253
Alexander T, Thiel A, Rosen O et al (2009) Depletion of autoreactive immunologic memory followed by autologous hematopoietic stem cell transplantation in patients with refractory SLE induces long-term remission through de novo generation of a juvenile and tolerant immune system. Blood 113:214–223
Atta EH, de Azevedo AM, Maiolino A et al (2009) High CD8+ lymphocyte dose in the autograft predicts early absolute lymphocyte count recovery after peripheral hematopoietic stem cell transplantation. Am J Hematol 84:21–28
Gergely L, Váncsa A, Miltényi Z et al (2011) Pretreatment T lymphocyte numbers are contributing to the prognostic significance of absolute lymphocyte numbers in B cell non-Hodgkin’s lymphomas. Pathol Oncol Res 17:249–255
Kaplan EL, Meier P (1958) Nonparametric estimation from incomplete observations. J Am Stat Assoc 53:457–481
Koehl U, Bochennek K, Zimmermann SY et al (2007) Immune recovery in children undergoing allogeneic stem cell transplantation: absolute CD8+CD3+ count reconstitution is associated with survival. Bone Marrow Transpl 39:269–278
McKinney EF, Lyons PA, Carr EJ et al (2010) A CD8+ T cell transcription signature predicts prognosis in autoimmune disease. Nat Med 16:586–591
Nikolov NP, Pavletic SZ (2008) Technology insight: hematopoietic stem cell transplantation for systemic rheumatic disease. Nat Clin Pract Rheumatol 4:184–191
Pace L, Tempez A, Arnold-Schrauf C et al (2012) Regulatory T cells increase the avidity of primary CD8+ T cell responses and promote memory. Science 338:532–536
Passweg J, Tyndall A (2007) Autologous stem cell transplantation in autoimmune diseases. Semin Hematol 44:278–285
Rutella S, Rumi C, Laurenti L et al (2000) Immune reconstitution after transplantation of autologous peripheral CD34+ cells: analysis of predictive factors and comparison with unselected progenitor transplants. Br J Haematol 108:105–115
Storek J, Geddes M, Khan F et al (2008) Reconstitution of the immune system after hematopoietic stem cell transplantation in humans. Semin Immunopathol 30:425–437
Szodoray P, Varoczy L, Szegedi G et al (2010) Autologous stem cell transplantation in autoimmune and rheumatic diseases: from the molecular background to clinical applications. Scand J Rheumatol 39:1–11
Szodoray P, Varoczy L, Papp G et al (2012) Immunological reconstitution after autologous stem cell transplantation in patients with refractory systemic autoimmune diseases. Scand J Rheumatol 41:110–115
Tyndall A, Saccardi R (2005) Haematopoietic stem cell transplantation in the treatment of severe autoimmune disease; results from phase I/II studies, prospective randomized trials and future directions. Clin Exp Immunol 141:1–9
Váróczy L, Kiss E, Tarr T et al (2012) Fatal CMV infection after autologous stem cell transplantation in refractory systemic lupus erythematosus. Case Rep Transpl 2012:465089
Williams KM, Gress RE (2008) Immune reconstitution and implications for immunotherapy following haematopoiteic stem cell transplantation. Best Pract Res Clin Haematol 21:579–596
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This work was supported by the grant of the Hungarian Research Fund OTKA 71883.
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Váróczy, L., Kovács, I., Baráth, S. et al. Changes in the Number of CD8+ T Lymphocytes in the Peripheral Blood of Patients with Various Autoimmune Diseases after Autologous Hematopoietic Stem Cell Transplantations and their Relations to the Survival Times. Arch. Immunol. Ther. Exp. 61, 421–426 (2013). https://doi.org/10.1007/s00005-013-0241-2
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DOI: https://doi.org/10.1007/s00005-013-0241-2