Abstract
We have identified a cDNA clone(CaEF1A) sharing significant homology with the protein synthesis elongation factor 1As (eEFIA) found in eukaryotes. This clone was isolated from a tobacco mosaic virus (TMV)-inoculatecl hot pepper(Capsicum annuum L.) cDNA library by differential screening. Based on the results of our Southern blot analysis using theCaEFIA full-length cDNA clone as a probe, we suggest thatCaEFIA exists as part of a multigene family in ~e hot pepper genome. In another experiment, the mRNA of theCaEFIA gene was found to accumulate in ripe fruits, flowers, young and old leaves, and roots, but was not detected in immature fruits or stems. The transcript ofCaEFIA accumula-tion was induced within 12 h of local wounding treatment, as well as by both avirulent and virulent TMV inoculations.
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Abbreviations
- eEFIA:
-
eukaryotic translation elongation factor-1A
- HR:
-
hypersensitive response
- ORF:
-
open reading frame
- PR:
-
pathogenesisrelated
- TMV:
-
tobacco mosaic virus
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The nucleotide sequence ofCaEFIA reported in this paper has been submitted to the EMBL, GenBank, and DDBJ under accession number AF242732.
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Shin, R., Park, CJ. & Paek, KH. A translation elongation factor 1A (CaEF1A) gene from hot pepper (Capsicum annuum L.) is induced by the tobacco mosaic virus and by wounding. J. Plant Biol. 44, 199–204 (2001). https://doi.org/10.1007/BF03030352
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DOI: https://doi.org/10.1007/BF03030352