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Zinc deficiency, ethanol, and myocardial ischemia affect lipoperoxidation in rats

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Abstract

The production of oxygen free radicals can be stimulated by excess iron, cadmium, nickel, and the like. Inversely, copper, zinc, and selenium inhibit production, either via their own action or via antiradical metalloenzymes. The study involved determining the effect of zinc deficiency combined with chronic ethanol administration on the status of blood and tissue free radicals, as well as on cardiac function in isolated perfused rats' hearts. Animals were fed a basic diet containing residual zinc at 0.2–0.3 ppm. Following a zinc deficiency lasting 5 wk, which during the last 4 wk was accompanied by chronic ethanol administration, hearts were submitted to ischemia for 30 min in vitro, followed by reperfusion. Biochemical analyses (zinc, superoxide dismutase, malondialdehyde, conjugated dienes, and so on) were performed in the blood and in the homogenates of different organs.

The experimental zinc deficiency caused a slight decrease of superoxide dismutase activity, accompanied by increased production of peroxidated lipids. Ethanol administration appeared to increase the levels of peroxidated lipids in the heart. Finally, the combination of zinc deficiency and ethanol administration had very harmful effects, especially on lipid peroxidation and contractile function of the isolated, perfused heart in preischemic conditions.

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Abbreviations

ADH:

aldehyde dehydrogenase

ALAT:

alanine aminotransferase

AP:

akaline phosphatase

ASAT:

aspartate aminotransferase

CPK:

creatine phosphokinase

CD:

conjugated dienes

CF:

coronary blood flow

γGT:

γ glutathion transpeptidase

GSH:

reduced glutathione

Gs=sG:

oxidized glutathione

GPx:

glutathione peroxidase

GRx:

glutathione reductase

Hb:

hemoglobin

HR:

heart rate

MDA:

malondialdehyde

OHP:

organic hydroperoxide

RBC:

red blood cells

SOD:

superoxide dismutase

XO:

xanthine oxidase

References

  1. E. S. Reynolds and M. T. Noslen,Free Radicals in Biology, Pryor W. A., ed., Academic, New York. 1980, p. 49–94.

    Google Scholar 

  2. W. T. Bettger, and B. L. O'Dell,Life Sci. 28, 1425–1438 (1981).

    Article  PubMed  CAS  Google Scholar 

  3. M. Chvapil, J. N. Ryan, and C. F. Zukoski,Proc. Soc. Exp. Biol. Med. 141, 150–153 (1972).

    PubMed  CAS  Google Scholar 

  4. M. Chvapil,Med. Clin. North Am. 60, 799–812 (1976).

    PubMed  CAS  Google Scholar 

  5. M. Chvapil, J. N. Ryan, and C. F. Zukoski,Proc. Soc. Exp. Biol. Med. 140, 642–646 (1972).

    PubMed  CAS  Google Scholar 

  6. J. F. Sullivan, M. S. Jetton, H. K. J. Hahn, and R. E. Burch,Am. J. Clin. Nutr.,33, 51–56 (1980).

    PubMed  CAS  Google Scholar 

  7. R. Nordmann, C. Ribiere, and H. Rouach,Prog. Clin. Biol. Res. 241, 201–213 (1987).

    PubMed  CAS  Google Scholar 

  8. N. Kocak-Toker, M. Uysal, G. Aykac, A. Sives, S. Yalcin, and H. Öz,Pharmacol. Res. Commun. 17, 233–239 (1985).

    Article  PubMed  CAS  Google Scholar 

  9. A. Valenzuela, N. Fernandez, V. Fernandez, G. Ugarte and L. A. Videla,FEBS Lett. 111, 11–13 (1980).

    Article  PubMed  CAS  Google Scholar 

  10. L. A. Videla, V. Fernandez, A. De Marinis, N. Fernandez, and A. Valenzuela,Biochem. Biophys. Res. Commun. 104, 965–970 (1982).

    Article  PubMed  CAS  Google Scholar 

  11. R. E. Litov, D. H. Irving, J. E. Downey, and A. L. Tappel,Lipids 13, 305–307 (1978).

    Article  PubMed  CAS  Google Scholar 

  12. S. Shaw, E. Jayatilleke, W. A. Ross, E. R. Gordon, and C. S. Lieber,J. Lab. Clin. Med. 98, 417–424 (1981).

    PubMed  CAS  Google Scholar 

  13. H. Speisky, D. Bunout, H. Orrego, H. G. Giles, A. Gunasekara, and Y. Israel,Res. Commun. Chem. Pathol. Pharmacol. 48, 77–90 (1985).

    PubMed  CAS  Google Scholar 

  14. I. E. Dreosti, Ciba foundation symposium 105. London, 103–123 (1984).

  15. I. E. Dreosti and E. J. Partick,Biol. Trace Elem. Res. 14, 179–190 (1987).

    Article  CAS  Google Scholar 

  16. H. A. Krebs and K. Henseleit,Hoppe Seylers Z. 210, 33–66 (1932).

    CAS  Google Scholar 

  17. O. Langendorff,Pflugers Arch. 61, 291–332 (1895).

    Article  Google Scholar 

  18. S. L. Marklund,J. Biol. Chem. 251, 7504–7507 (1976).

    PubMed  CAS  Google Scholar 

  19. K. Yagi,Biochem. Res. 15, 212–216 (1976).

    CAS  Google Scholar 

  20. H. Ohkawa, N. Ohishi, and K. Yagi,Anal. Biochem. 95, 351–358 (1979).

    Article  PubMed  CAS  Google Scholar 

  21. D. Wickens, M. H. Wilkins, J. Lunec, G. Ball, and T. L. Dormandy,Ann. Clin. Biochem. 18, 158–162 (1981).

    PubMed  CAS  Google Scholar 

  22. R. L. Heath and A. L. Tappel,Anal. Biochem. 76, 184–191 (1976).

    Article  PubMed  CAS  Google Scholar 

  23. W. A. Gunzler, H. Kremers, and L. Flohe,Z. Klin. Chem. Klin. Biochem. 10, 444–448 (1974).

    Google Scholar 

  24. I. Carlberg and M. Mannervik,Methods Enzymol. 113, 484–490 (1985).

    Article  PubMed  CAS  Google Scholar 

  25. R. R. Beers and I. W. Sizer,J. Biol. Chem. 195, 133–135 (1952).

    PubMed  CAS  Google Scholar 

  26. F. Tietze,Anal. Biochem. 27, 502–522 (1969).

    Article  PubMed  CAS  Google Scholar 

  27. I. D. Desai,Methods Enzymol. 105, 138–147 (1984).

    Article  PubMed  CAS  Google Scholar 

  28. M. Sugiura, K. Kato, T. Adachi, Y. Ito, and K. Hirano,Chem. Pharm. Bull. 29, 430–432 (1981).

    PubMed  CAS  Google Scholar 

  29. D. C. Salo, S. W. Lin, R. F. Pacifier, and K. J. T. Davies,Free Radical Biol. Med. 5, 335–339 (1988).

    Article  CAS  Google Scholar 

  30. H. W. Sippel,Acta Pharmacol. Toxicol. 53, 135–140 (1983).

    CAS  Google Scholar 

  31. S. Morton and M. C. Mitchell,Biochem. Pharmacol. 34, 1559–1563 (1985).

    Article  PubMed  CAS  Google Scholar 

  32. B. Mills, R. D. Lindeman, and C. A. Lang,J. Nutr. 111, 1098–1102 (1981).

    PubMed  CAS  Google Scholar 

  33. J. C. Seagrave, R. A. Tobey, and C. D. Hildebrand,Biochem. Pharmacol. 32, 3017–3021 (1983).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biochimie C, Centre Hospitalier Régional de Grenoble, BP 217 X, 38043, Grenoble Cédex, France

    Charles Coudray, Marie J. Richard, Josiane Arnaud & Alain Favier

  2. Laboratoire de Physiologie Cellulaire Cardiaque, URA CNRS 632, Université Joseph Fourier, 38041, Grenoble Cédex, France

    François Boucher & Joel De Leiris

Authors
  1. Charles Coudray
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  2. François Boucher
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  3. Marie J. Richard
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  4. Josiane Arnaud
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  5. Joel De Leiris
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  6. Alain Favier
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Coudray, C., Boucher, F., Richard, M.J. et al. Zinc deficiency, ethanol, and myocardial ischemia affect lipoperoxidation in rats. Biol Trace Elem Res 30, 103–118 (1991). https://doi.org/10.1007/BF02990347

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  • DOI: https://doi.org/10.1007/BF02990347

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