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Determination of seedborne fungi and detection of aflatoxins in sudanese fenugreek seeds

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Abstract

Following incubation of fenugreek(Trigonella foenum- graecum L.) seeds on potato dextrose agar and moist filter papers at 28±2°C, 59 species and 11 varieties belonging to 21 genera of fungi were determined as seedborne in fenugreek crops. Among these isolates, 45 species and 9 varieties are new records for this crop; and two species are new to the mycoflora of Sudan:Aspergillus stellifer andEmericella variecolor. The genusAspergillus (15 species and 8 varieties) is the most prevalent, followed byDrechslera (3 species),Rhizopus (3 species),Alternaria andFusarium (6 species each),Emericella (4 species and 2 varieties)Cladosporium andPenicillium (4 species each),Chaetomium (3 species) andCurvularia (3 species and one variety). The remaining 11 genera displayed low level of infection. Of the common pathogens of fenugreek plants,Fusarium oxysporum (2.13%) was recovered from the seeds of this crop. Thin layer Chromatographic analysis of chloroform extracts of 13 seed samples showed that two samples were naturally contaminated with aflatoxins B1, B2, G1 and G2 (7.5–35.2 µg/kg).

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Correspondence to S. A. F. El-Nagerabi.

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http://www.phytoparasitica.org posting Nov. 4, 2001.

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El-Nagerabi, S.A.F. Determination of seedborne fungi and detection of aflatoxins in sudanese fenugreek seeds. Phytoparasitica 30, 61–66 (2002). https://doi.org/10.1007/BF02983971

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  • DOI: https://doi.org/10.1007/BF02983971

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