Abstract
We previously demonstrated the ability of ginseng saponins (active ingredients ofPanax ginseng) to enhance Ca2+-activated Cl∼ current. The mechanism for this ginseng saponin-induced enhancement was proposed to be the release of Ca2+ from IP3-sensitive intracellular stores through the activation of PTX-insensitive Gαa/11, proteins and PLC pathway. Recent studies have shown that calmodulin (CaM) regulates IP3 receptor-mediated Ca2+ release in both Ca2+-dependent and -independent manner. In the present study, we have investigated the effects of CaM on ginseng saponin-induced Ca2+-activated CI-current responses inXenopus oocytes. Intraoocyte injection of CaM inhibited ginseng saponin-induced Ca2+-activated CI-current enhancement, whereas co-injection of calmidazolium, a CaM antagonist, with CaM blocked CaM action. The inhibitory effect of CaM on ginseng saponin-induced Ca2+-activated CI-current enhancement was dose- and time-dependent, with an IC50 of 14.9 ± 3.5 μM. The inhibitory effect of CaM on saponin’s activity was maximal after 6 h of intraoocyte injection of CaM, and after 48 h the activity of saponin recovered to control level. The half-recovery time was calculated to be 16.7 ± 4.3 h. Intraoocyte injection of CaM inhibited Ca2+-induced Ca2+-activated CI-current enhancement and also attenuated IP3-induced Ca2+-activated CI-current enhancement. Ca2+/CaM kinase II inhibitor did not inhibit CaM-caused attenuation of ginseng saponin-induced Ca2+-activated CI-current enhancement. These results suggest that CaM regulates ginseng saponin effect on Ca2+-activated CI-current enhancementvia Ca2+-independent manner.
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Adkins, C. E., Morris, S. A., De Smedt, H., Sienaert, I., Torok, K., and Taylor, C. W., Ca2+-calmodulin inhibit Ca2+ release mediated by type-1, -2, and -3 inositol trisphosphate receptors.Biochem. J., 345, 357–363 (2000).
Berridge, M. J. and Irvine, R. F., Inositol triphosphates and cell signalling.Nature, 341,197–205 (1989).
Berridge, M. J., Bootman, M. D., and Lipp, P., Calcium — a life and death signal.Nature (Lond.), 395,645–648 (1998).
Cardy, T. J. A. and Taylor, C. W., A novel role for calmodulin: Ca2+-independent inhibition of type-1 inositol trisphosphate receptors.Biochem. J., 334,447–455 (1998).
Choi, S., Rho, S. H., Jung, S. Y., Kim, S. C., Park, C. S., and Nah, S. Y., A novel activation of Ca2+-activated CI-channel inXenopus oocytes by ginseng saponins: evidence for the involvement of phospholipase C and intracellular Ca2+ mobilization.Br. J. Pharmacol., 132,641–648 (2001a).
Choi, S., Kim, H. J., Ko, Y. S., Jeong, S. W., Kim, Y. l., Simonds, W. R., Oh, J. W., and Nah, S. Y., Gαa/11 coupled to mammalian phospholipase C b3-like enzyme mediates the ginsenoside effect on Ca2+-activated CI-current in theXenopus oocyte.J. Biol. Chem., 276,48797–48802 (2001b).
Dascal, N., Yekuel, R., and Oron, Y., Acetylcholine promotes progesterone-induced maturation ofXenopus oocytes.J. Exp. Zool., 230,131–135 (1984).
Fukunaga, K., Miyamoto, E., and Soderling T. R., Regulation of Ca2+/calmodulin-dependent protein kinase II by brain gangliosides.J. Neurochem., 54,103–109 (1990).
Gnegy, M. E., Calmodulin in neurotransmitter and hormone action.Ann. Rev. Pharmacol. Toxicol., 33,45–70 (1993).
Hartzell, H. C., Activation of different CI-currents inXenopus oocytes by Ca liberation from stores and by capacitative Ca Influx.J. Gen. Physiol., 108,157–175 (1996).
Jeong, S. M., Lee, J. H., Kim, S., Rhim, H., Lee B. H., Kim, J. H., Oh, J. W., and Lee, S. M., Ginseng saponins induce store-operated calcium entry inXenopus oocytes.Br. J. Pharmacol., 142,585–593 (2004).
Kasri, N. N., Bultynck, G., Sienaert, I., Callewaert, G., Erneux, C., Missiaen, L., Pary, J. B., and De Smedt, H., The role of calmodulin for inositol 1,4,5-trisphosphate receptor function.Biochim. Biophys. Acta, 1600,19–31 (2002).
Kuruma, A. and Hartzell, H. C., Dynamics of calcium regulation of chloride currents inXenopus oocytes.Am. J. Physiol., 276, C161-C175(1999).
Lechleiter, J.D. and Clapham, D. E., Molecular mechanisms of intracellular calcium excitability inXenopus laevis oocytes.Cell, 69, 283–294 (1992).
Lee, J. H., Jeong, S. M., Lee, B. H., Kim, D. H., Kim, J. H., Kim, J. I., and Nah, S. Y., Prevention of ginsenoside-induced desensitization of Ca2+-activated CI-currents by microinjection of inositol hexakisphosphate (lnsP6) inXenopus laevis oocytes: involvement of GRK2 and β-arrestin I.J. Biol. Chem., 279, 9912–9921 (2004).
Liu, M., Chen, T. Y., Ahamed, B., Li, J., and Yau, K. W., Calcium-calmodulin modulation of the olfactory cyclic nucleotidegated cation channel.Science, 266,1348–1354 (1994).
Matifat, R., Fournier, F., Lorca, T., Capony, J. P., Brule, G., and Collin, T., Involvement of the Ca2+/calmodulin protein kinase II pathway in the Ca2+-mediated regulation of the capacitative Ca2+ entry inXenopus oocytes.Biochem. J., 322, 267–272 (1997).
Missiaen, L., Parys, J. B., Weidema, A. R., Sipma, H., Valingen, S., De Smet, P., Callewaert, G., and De Smedt, H., The bell-shaped Ca2+ dependence of the inositol 1,4,5-trisphosphate-induced Ca2+ release is modulated by Ca2+calmodulin.J. Biol. Chem., 214, 13748–13751 (1999).
Nah, S. Y., Ginseng, recent advances and trend.Korea J. Ginseng Sci., 21,1–12 (1997).
Parekh A. B., Interaction between capacitative Ca2+ influx and Ca2+-dependent Cl-currents inXenopus oocytes.Pflugers Arch-Eur. J. Physiol., 430, 954–963 (1995).
Parekh, A. B. and Penner, R., Store depletion and calcium influx.Physiol. Rev., 77, 901–930 (1997).
Parys, J. B., Sernett, S. W., DeLisle, S., Snyder, P. M., Welsh, M. J., and Campbell, K. P., Isolation, characterization, and localization of the inositol 1,4,5-triphosphate receptor protein in Xenopus laevis oocytes.J. Biol. Chem., 267,18776–18782 (1992).
Sienaert, I., Kasri, N. N., Vanlingen, S., Parys, J. B., Callewaert, G., Messeian, L., and de Smedt, L., Localization and function of a calmodulin/apocalmodulin binding domain in the N-terminal part of the type-1 inositol 1,4,5-trisphosphate receptor.Biochem. J., 365,269–277 (2002).
Taylor, C. W. and Laude, A. J., IP3 receptors and their regulation by calmodulin and cytosolic Ca2+.Cell Calcium, 32, 321–334 (2002).
Yamada, M., Miyawaki, A., Saito, K., Nakajima, M., Yamamoto-Hino, Y., Ryo, T., Furuichi, T., and Mikoshiba, K., The calmodulin-binding domain in the mouse type 1 inositol 1,4,5- trisphosphate receptor.Biochem. J., 308,83–88 (1995).
Waxham, M. N. and Aronowski, J., Ca2+/calmodulin-dependent protein kinase II is phosphorylated by protein kinase Cin vitro.Biochemistry, 32,2923–2930 (1993).
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Lee, JH., Jeong, SM., Lee, BH. et al. Effect of calmodulin on ginseng saponin-induced Ca2+-Activated CI-channel activation inXenopus laevis oocytes. Arch Pharm Res 28, 413–420 (2005). https://doi.org/10.1007/BF02977670
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DOI: https://doi.org/10.1007/BF02977670