Skip to main content
Log in

Whole broth cellulase production for use in simultaneous saccharification and fermentation

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Cellulase, an enzyme that catalyzes the breakdown of cellulose into glucose, is produced inside fungal cells and secreted into the surrounding media. However, the cells retain significant amounts of β-glucosidase, and this extra enzyme could improve the performance of a simultaneous saccharification and fermentation (SSF) process. To test the possible contribution of mycelia-bound enzyme to SSF performance with modern fungal cellulase producers, the performance of SSF using whole culture broth (with cells) was compared to SSF performance using culture filtrate. Cellulase broth was produced using the advancedTrichoderma reesei mutant L27. Measurements of cellulase activities showed that the broth had more activity than the filtrate. Subsequent SSF experiments were conducted at 37°C in shake flasks withSaccharomyces cerevisiae, and the broth produced 8–25% higher ethanol yields than the cellulase filtrate produced.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wright, J. D. (1987),Chem. Eng. Prog. 84, 62.

    Google Scholar 

  2. Hinman, N. D., Spindler, D., Wright, J. D., Grohmann, K., and Wyman, C. E. (1988), “Cellulase Production: An Economic Analysis,” draft, Solar Energy Research Institute, Golden, CO.

    Google Scholar 

  3. Spindler, D. D., Wyman, C. E., Grohmann, K. and Mohagheghi, A. (1989),Appl. Biochem. Biotech. 20/21, 529.

    Google Scholar 

  4. Acebal, C., Castillon, M. P., and Estrada, P. (1988),Biotech. Appl. Biochem. 10, 1.

    CAS  Google Scholar 

  5. Takagi, M., Abe, S., Suzuki, S., Emert, G. H., and Yata, N.(1977),Proceedings Bioconversion Symp., Ghose, T K., ed., IIT, New Dehli, India, 551.

    Google Scholar 

  6. Mohagheghi, A., Grohmann, K., and Wyman, C. E. (1988),Appl. Biochem. Biotech. 17, 263.

    Article  CAS  Google Scholar 

  7. Spindler, D. D., Wyman, C. E., Mohagheghi, A., and Grohmann, K. (1987),Appl. Biochem. Biotech. 17, 279.

    Article  Google Scholar 

  8. Glose, T. K. (1987),Pure Appl. Chem. 59, 257.

    Article  Google Scholar 

  9. Tatsumoto, K., Baker, J. O., Tucker, M. P., Oh, K. K., Mohagheghi, A., Grohmann, K., and Himmel, M. E. (1987),Appl. Biochem. Biotech. 18, 159.

    Article  Google Scholar 

  10. Andreotti, R. E., Mandels, M., and Roche, C. (1977),Proceedings Bioconversion Symp., Ghose, T. K., ed., IIT, New Dehli, India, 249.

    Google Scholar 

  11. Mukhopadhyay, S. N., and Malik, R. K. (1980),Biotech. Bioeng. 22, 2237.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schell, D.J., Hinman, N.D., Wyman, C.E. et al. Whole broth cellulase production for use in simultaneous saccharification and fermentation. Appl Biochem Biotechnol 24, 287–297 (1990). https://doi.org/10.1007/BF02920253

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02920253

Index Entries

Navigation