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Detection of a simplex RAPD marker linked to resistance to potato virus Y in a tetraploid potato

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Abstract

Extreme resistance to potato virus Y, derived from a wild diploid speciesSolanum chacoense, was found in Japanese cultivar Konafubuki. The segregation ratio of resistant vs susceptible in the tetraploid population from Kita-akari (susceptible) x Konafubuki (resistant) indicated that the resistance gene followed a monogenic dominant fashion. Bulked DNA samples of resistant and of susceptible clones were screened with 306 decamer primers by PCR to find RAPD markers linked to the resistance. The RAPD marker 38-530 was reproducibly detected in the resistant clones with a recombination frequency of 16.3%. Except for Konafubuki the marker band was found only in a few limited parental lines and cultivars where the resistance is not involved. Thus, using Konafubuki as a resistance gene source, the RAPD marker 38-530 would be practically and widely useful in tetraploid breeding programs.

Resumen

En el cultivar japonés “Konafubuki” se encontró resistencia extrema al virus de la papa Y, derivada de una especie silvestre diploide deSolanum chacoense. El rango de segregatión de la resistencia vs. la susceptibilidad en la población tetraploide de “Kita-akari” (susceptible) x “Konafubuki” (resistente) indicó que el gen de resistencia siguió un patrón dominante monogénico. Muestras agrupadas de mezclas de ADN de resistencia y de clones susceptibles fueron tamizadas usando 306 iniciadores decaméricos por PCR (Reacción on cadena de la polimerasa) para encontrar marcadores de RAPD vlnculados a la resistencia Se descubrió cierto grado de reproduction en clones resistentes del marcador RAPD 38-530 con una frecuencia de recombinatión del 16.3%. A exception de “Konafubuki”, la banda del marcador se encontró sólo en unas pocas lineas parentales y cultivares donde no estaba implicada la resistencia.. Así, usando “Konafubuki” como fuente genética de restencia, el marcador RAPD 38-530 sería muy útil en los programas de mejoramiento de tetraploides.

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Authors and Affiliations

  1. Experimental Farm, Kobe University, 1348 Uzurano, Kasai, 675-2103, Hyogo, Japan

    Kazuyoshi Hosaka & Yuko Hosaka

  2. Hokkaido National Agricultural Experiment Station, Shinsei, Memuro, Kasai, 082-0071, Hokkaido, Japan

    Motoyuki Mori

  3. Unzen Station, National Center for Seeds and Seedlings, 1494-34, Saigo Bo, Mizuho, Minamitakaki, 859-1211, Nagasaki, Japan

    Tsutomu Maida

  4. Hokkaido Prefectural Tokachi Agricultural Experiment Station, Shinsei, Memuro, Kasai, 082-0071, Hokkaido, Japan

    Hiroshi Matsunaga

Authors
  1. Kazuyoshi Hosaka
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  2. Yuko Hosaka
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  3. Motoyuki Mori
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  4. Tsutomu Maida
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  5. Hiroshi Matsunaga
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Correspondence to Kazuyoshi Hosaka.

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Hosaka, K., Hosaka, Y., Mori, M. et al. Detection of a simplex RAPD marker linked to resistance to potato virus Y in a tetraploid potato. Am. J. Pot Res 78, 191–196 (2001). https://doi.org/10.1007/BF02883544

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