Abstract
Orotic acid in 0.1% concentration influenced the rate of growth ofRhodotorula gracilis in relation to the source of nitrogen nutrients. The maximum stimulation of growth (150%) was obtained on mineral medium containing asparagine. Mild stimulation of growth was also manifested in the phase of fat production. The accumulation of fat in the yeast cells was not influenced by orotic acid, but the rate of its synthesis was mildly stimulated together with growth. This phenomenon is discussed from the aspect of the effect of orotic acid on metabolic processes.
Abstract
Оротовая кислота в концентрации 0,1% влияет на скорость роста дрожжей Rhodotorula gracilis в зависимости от источника азота. Максимальное ускорение роста, полученное на минеральной среде с аспарагином, составляло 150%. Некоторое усиление роста наблюдалось также в фазе жирообразования. Оротовая кислота не влияет на аккумуляцию жира в клетках дрожжей, но скорость его синтеза несколько стимулирует вместе с ростом.— Это явление обсуждается с точки зрения влияния оротовой кислоты на процессы обмена веществ.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chargaff, E., Davidson, J. N.:The nucleic acids II, p. 299. Academic Press, New York, 1955.
Creasey, W. A., Hankin, L., Handschumacher, R. E.:Fatty livers induced by orotic acid. I. Accumulation and metabolism of lipids. J. Biol. Chem. 236: 2064, 1961.
Enebo, L., Anderson, L. G., Lundin, H.:Microbiological fat synthesis by means of Rhodotorula yeast. Arch. Biochem. 11: 383, 1946.
Hoagland, M. B.:Enzymatic reactions between amino acids and ribonucleic acids as intermediate steps in protein synthesis. Prov. Fourth Intern. Cong. Biochem., Vienna, 8: 199, 1958.
Kleinzeller, A., Bass, A.:Biosynthesa tuků ze saccharidů v kvasinkách I. Složení tuků v různých fázích tworby tuku. Chem. listy 45: 303, 1951.
Kleinzeller, A., Málek, I., Praus, R., Škoda, J.:Biosynthesa tuků ze saccharidů v kvasinkách III. Tvorba tuků z d-xylosy a glukosy v kvasince Rhodotorula gracilis. Chem. listy 46: 470, 1952.
Lieberman, I., Kornberg, A., Simms, E. S.:Enzymatic synthesis of pyrimidine nucleotides. Orotidine-5′-phosphate. J. Biol. Chem. 215: 403, 1955.
Marchetti, M., Caldarera, C. M., Moruzzi, G.:Orotic acid and nucleotide metabolism in the liver of vitamin B 12-deficient chicks. It. J. Biochem. 9: 371, 1960.
Marchetti, M., Caldarera, C. M.:Relationship between orotic acid and vitamin B 12 in nucleotide metabolism. It. J. Biochem. 7: 27, 1963.
Moruzzi, G., Marchetti, M., Vivani, R.:Effect of orotic acid on the synthesis of citrovorum factor and of methionine in liver of the chick. Nature 199: 695, 1963.
Praus, R.:Studie biosynthesy karotenoidů v kvasince Rhodotorula gracilis II. Vliv svêtla na tvorbu a obsah karotenoidů. Chem. listy 46: 643, 1952.
Praus, R., Dyr, J.:Biosynthesa karotenoidů v kvasince Rhodotorula gracilis V. Bezbarvé polyeny. Chem. listy 51: 1559, 1957.
Standerfer, S. B., Handler, P.:Fatty liver induced by orotic acid feeding. Proc. Soc. exp. Biol. (N. Y.) 90: 270, 1955.
Škoda, J.:Studie biosynthesy karotenoidů v Rhodotorula gracilis I. Isolace torulenu a vliv kultivační teploty na pomêrné množství torulenu k ostatním karotenoidům Rhodotoruly gracilis. Chem. listy 45: 413, 1951.
Škoda, J., Šlechta, L.:Acidobutyrometrické stanovení tuku v tukotvorných kvasinkách. Chem. listy 49: 1097, 1955.
Woodbine, M.:Microbial fat: Microorganisms as potential fat producers. Progress in Industrial Microbiology 1: 179, 1959.
Yamada, K., Kowaguchi, K.:Fat deposition in the kidney and liver by the administration of orotic acid. Vitamins 22: 168, 1961.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Řeháček, J., Beran, K. Effect of orotic acid on growth and fat synthesis in the yeastRhodotorula gracilis . Folia Microbiol 9, 214–217 (1964). https://doi.org/10.1007/BF02875839
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02875839