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The mouseCD7 gene: Identification of a new element common to the humanCD7 and mouseThy-1 promoters

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Abstract

HumanCD7 (CD7) is a 40000M r member of the immunoglobulin gene superfamily that is expressed early in natural killer (NK) and T-lymphocyte development.CD7 is involved in lymphocyte activation, as ligation ofCD7 activates NK and TCRγδ T lymphocytes, and ligation ofCD7 on TCRαβ T lymphocytes induces a non-mitogenic calcium flux. We have previously cloned and characterized the gene for humanCD7 (hCDT) and have described its expression in transgenic mice. Recently a mouse cDNA homologous tohCD7 was reported, which we mapped to the corresponding mouse chromosomal location ashCD7. We now report the identification and characterization of a mouseCD7 (mCDT) genomic clone. We demonstrated that themCD7 gene was similar both in size and structural organization tohCD7. Comparison of the 5′ flanking sequences of themCD7 andhCD7 genes revealed two regions of sequence similarity. Electrophoretic mobility shift assay confirmed both of these regions to be sites of tissue-restricted protein binding in vitro. The more 3′ similarity region also shared sequence with a region in the mouseThy-1 gene 5′ flanking region, suggesting that this sequence may be a cis-acting regulatory element common to all three genes. Thus, the promoter regions and exonic organization were similar in the humanCDT, mouseCDT, and mouseThy-1 genes.

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Authors and Affiliations

  1. Departments of Immunology, Pediatrics, Medicine, Biochemistry, Duke University Medical Center, 27710, Durham, North Carolina, USA

    David M. Lee, Laura E. Schanberg, Donald E. Fleenor, Michael F. Seldin, Barton F. Haynes & Russel E. Kaufman

  2. Duke Comprehensive Arthritis and Cancer Centers, Duke University Medical Center, 27710, Durham, North Carolina, USA

    David M. Lee, Laura E. Schanberg, Donald E. Fleenor, Michael F. Seldin, Barton F. Haynes & Russel E. Kaufman

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  1. David M. Lee
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  2. Laura E. Schanberg
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  3. Donald E. Fleenor
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  4. Michael F. Seldin
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  5. Barton F. Haynes
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  6. Russel E. Kaufman
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Additional information

The nucleotide sequence data reported in thts paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number U23462

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Lee, D.M., Schanberg, L.E., Fleenor, D.E. et al. The mouseCD7 gene: Identification of a new element common to the humanCD7 and mouseThy-1 promoters. Immunogenetics 44, 108–114 (1996). https://doi.org/10.1007/BF02660058

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  • DOI: https://doi.org/10.1007/BF02660058

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