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Identification of new TAP2 alleles in gorilla: evolution of the locus within hominoids

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Abstract

Transporters associated with antigen processing molecules (TAP1 and TAP2) mediate the transfer of cytosolic peptides into the lumen of the endoplasmic reticulum for association with newly synthesized class I molecules of the major histocompatibility complex. Previous molecular and functional analyses of rat and humanTAP2 homologues indicated major differences in gene diversification patterns and selectivity of peptides transported. Therefore, in this study, we analyzed the alleles of the gorillaTAP2 locus to determine whether the pattern of diversification resembled that in either of those two species. Sequence analysis of theTAP2 cDNAs from gorilla Epstein-Barr virus-transformed B-cell lines revealed four alleles with a genetic distance of less than 1%. The nucleotide substitutions distinguishing the alleles are confined to the 3′ half of the coding region and occur individually or within two small clusters of variability. Diversification of the locus appears to have resulted from point substitutions and recombinational events. Evolutionary-rate estimates for theTAP2 gene in gorilla and human closely approximate those observed for other hominoid genes. The amino acid polymorphisms within the gorilla molecules are distinct from those in the human homologues. The absence of ancestral polymorphisms suggests that gorilla and humanTAP2 genes have not evolved in a trans-species fashion but rather have diversified since the divergence of the lineages.

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Authors and Affiliations

  1. Department of Immunology, The University of Texas M.D. Andreson Cancer Center, 1515 Holcombe Blvd., Box 180, 77030, Houston, TX, USA

    Paul T. Loflin, Purnima R. Laud & David A. Lawlor

  2. Wiscoasin Regional Primate Research Center and Department of Pathology, University of Wisconsin, 1220 Capitol Court, 53715, Madison, WI, USA

    David I. Watkins

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  1. Paul T. Loflin
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  2. Purnima R. Laud
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  3. David I. Watkins
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  4. David A. Lawlor
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Additional information

The nucleotide sequence data reported in this paper have been submitted to the EMBL/GenBank nucleotide sequence databases and have been assigned the accession numbers L49032 (Gogo-TAP2a), L49033 (Gogo-TAP2b), L49034 (Gogo-TAP2c), and L49035 (Gogo-TAP2d)

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Loflin, P.T., Laud, P.R., Watkins, D.I. et al. Identification of new TAP2 alleles in gorilla: evolution of the locus within hominoids. Immunogenetics 44, 161–169 (1996). https://doi.org/10.1007/BF02602581

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  • DOI: https://doi.org/10.1007/BF02602581

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