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Structure of aGelasinospora linear plasmid closely related to the kalilo plasmid ofNeurospora intermedia

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Abstract

We have determined the complete nucleotide sequence of a linear mitochondrial plasmid from a natural isolate of a homothallic species ofGelasinospora. The plasmid genome is 8231 by long. It carries terminal inverted repeats of 1137 bp. Extending inwards from the terminal repeats are two long open reading frames coding for putative proteins with similarity to DNA and RNA polymerases. These are separated by a short intergenic region. The plasmid sequence shows remarkable similarity to that of theNeurospora intermedia senescence-plasmid kalilo. Overall the two plasmids have a similar genetic organization and are clearly homologous at the sequence level. The main differences are in the intergenic region and in the terminal repeats.

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References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment research tool. J Mol Biol 215:403–410

    Article  PubMed  Google Scholar 

  • Arganoza MT, Min J, Hu Z, Akins RA (1994) Distribution of seven homology groups of mitochondrial plasmids inNeurospora:evidence for widespread mobility between species in nature. Curr Genet 26:62–73

    Article  PubMed  Google Scholar 

  • Arx JA von (1982) A key to the species ofGelasinospora. Persoonia 11:443–449

    Google Scholar 

  • Bertrand H, Griffiths AJF (1989) Linear plasmids that integrate into mitochondrial DNA in Neurospora. Genome 31:155–159

    Google Scholar 

  • Bertrand H, Chan BS-S, Griffiths AJF (1985) Insertion of a foreign nucleotide sequence into mitochondrial DNA causes senescence inNeurospora intermedia. Cell 41:877–844

    Article  PubMed  Google Scholar 

  • Chan BS-S, Court DA, Vierula PJ, Bertrand H (1991) The kalilo linear senescence-inducing plasmid ofNeurospora is an invertron and encodes DNA and RNA polymerases. Curr Genet 20:225–237

    Article  PubMed  Google Scholar 

  • Collins RA, Saville BJ (1990) Independent transfer of mitochondrial chromosomes and plasmids during unstable vegetative fusion inNeurospora. Nature 345:177–179

    Article  PubMed  Google Scholar 

  • Court DA, Bertrand H (1991) A method for sequencing uncloned termini of linear plasmids. Nucleic Acids Res 19:1714

    PubMed  Google Scholar 

  • Court DA, Bertrand H (1992) Genetic organization and structural features of maranhar, a senescence-inducing linear mitochondrial plasmid ofNeurospora crassa. Curr Genet 22:385–397

    Article  PubMed  Google Scholar 

  • Court DA, Bertrand H (1993) Expression of the open reading frames of a senescence-inducing linear mitochondrial plasmid ofNeurospora crassa. Plasmid 30:51–66

    Article  PubMed  Google Scholar 

  • Court DA, Griffiths AJF, Kraus SR, Russell PJ, Bertrand H (1991) A new senescence-inducing linear plasmid in field-isolatedNeurospora crassa strains from India. Curr Genet 19:129–137

    Article  PubMed  Google Scholar 

  • Davis RH, DeSerres FJ (1970) Genetic and microbiological techniques forNeurospora crassa. Methods Enzymol 17A:79–143

    Google Scholar 

  • Debets F, Yang X, Griffiths AJF (1994) Vegetative incompatibility in Neurospora:its effect on horizontal transmission of mitochondrial plasmids and senescence in natural populations. Curr Genet 26:113–119

    Article  PubMed  Google Scholar 

  • Debets F, Yang X, Griffiths AJF (1995) The dynamics of mitochondrial plasmids in a Hawaiian population ofNeurospora intermedia. Curr Genet (in press)

  • Esser K, Kuck U, Lang-Hinrichs C, Lemke P, Osiewacz HD, Stahl U, Tudzynski P (1986) Plasmids of eukaryotes. Fundamentals and applications. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Fuller MS, Jaworski A (eds) (1987) Zoosporic fungi in teaching and research. Southeastern Publishing Corporation, Athens, Georgia

    Google Scholar 

  • Glass NL, Metzenberg RL, Raju NB (1990) Homothallic Sordariaceae from nature; the absence of strains containing only the a mating-type sequence. Exp Mycol 14:274–289

    Google Scholar 

  • Griffiths AJF (1992) Fungal senescence. Annu Rev Genet 26:351–372

    Article  PubMed  Google Scholar 

  • Griffiths AJF, Bertrand H (1984) Unstable cytoplasms in Hawaiian strains ofNeurospora intermedia. Curr Genet 8:387–398

    Article  Google Scholar 

  • Griffiths AJF, Kraus SR, Barton R, Court DA, Myers CJ, Bertrand H (1990) Heterokaryotic transmission of senescence plasmid DNA inNeurospora. Curr Genet 17:139–145

    Article  Google Scholar 

  • Griffiths AJF, Yang X, Barton R, Myers CJ (1992) Suppression of cytoplasmic senescence inNeurospora. Curr Genet 21:479–484

    Article  PubMed  Google Scholar 

  • Henikoff S, Eghtedarzadeh MK (1987) Conserved arrangement of nested genes at theDrosophila Gart locus. Genetics 117:711–725

    PubMed  Google Scholar 

  • Hermanns J, Osiewacz HD (1992) The linear mitochondrial plasmid pAL2-1 of a long-livedPodospora anserina mutant is an invertron encoding a DNA and RNA polymerase. nn 22:491–500

    Google Scholar 

  • Kempken F (1995) Horizontal gene transfer of a mitochondrial plasmid. Mol Gen Genet (in press)

  • Kempken F, Hermanns J, Osiewacz HD (1992)Evolution of linear plasmids. J Mol Evol 35:502–513

    Article  PubMed  Google Scholar 

  • Lambowitz A (1979) Preparation and analysis of mitochondrial ribosomes. Methods Enzymol 59:421–433

    PubMed  Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Marcinko-Kuehn M, Yang X, Debets F, Jacobson JJ, Griffiths AJF (1994) A kalilo -like linear plasmid in Louisiana field isolates of the pseudohomothallic fungusNeurospora tetrasperma Curr Genet 26:336–343

    Article  PubMed  Google Scholar 

  • Meinhardt F, Kempken F, Kamper K, Esser K (1990) Linear plasmids among eukaryotes: fundamentals and applications. Curr Genet 17:89–95

    Article  PubMed  Google Scholar 

  • Myers CJ, Griffiths AJF, Bertrand H (1989) Linear kalilo DNA is aNeurospora mitochondrial plasmid that integrates into the mitochondrial DNA. Mol Gen Genet 220:113–120

    Article  PubMed  Google Scholar 

  • Natvig DO, May G, Taylor JW (1984) Distribution and evolutionary significance of mitochondrial plasmids inNeurospora spp. J Bacteriol 159:288–293

    PubMed  Google Scholar 

  • Oeser B, Tudzynski P (1989) The linear mitochondrial plasmid pCIKI of the phytopathogenic fungusClaviceps purpurea may code for a DNA polymerase and an RNA polymerase. Mol Gen Genet 217:132–140

    Article  PubMed  Google Scholar 

  • Rohe M, Schrage K, Meinhardt F (1991) The linear plasmid pMC3-2 fromMorchella conica is structurally related to adenoviruses. Curr Genet 20:527–533

    Article  PubMed  Google Scholar 

  • Sakaguchi K (1990) Invertrons, a class of structurally and functionally related genetic elements that includes linear DNA plasmids, transposable elements, and genomes of adenotype viruses. Microbiol Rev 54:66–74

    PubMed  Google Scholar 

  • Taylor JW, Smolich BD, May G (1985) An evolutionary comparison of homologous mitochondrial plasmid DNAs from threeNeurospora species. Mol Gen Genet 201:161–167

    Article  PubMed  Google Scholar 

  • Taylor JW, Bruns TD, White TJ (1990) Unusual rate of molecular evolution in the ribosomal DNA internal transcribed spacer region of Sordariaceae? Mycol Soc Am Newslett 41:40–41

    Google Scholar 

  • Vickery DB, Griffiths AJF (1993) Transcription of the kalilo linear senescence plasmid fromNeurospora intermedia. Plasmid 29:180–192

    Article  PubMed  Google Scholar 

  • Vierula PJ, Cheng CK, Court DA, Humphreys RW, Thomas DY, Bertrand H (1990) The kalilo senescence plasmid ofNeurospora intermedia has covalently-linked 5′ terminal proteins. Curr Genet 17:195–201

    Article  Google Scholar 

  • Yang X, Griffiths AJF (1993a) Plasmid diversity in senescent and non-senescent strains ofNeurospora. Mol Gen Genet 237:177–186

    Article  PubMed  Google Scholar 

  • Yang X, Griffiths AJF (1993b) Plasmid suppressors active in the sexual cycle ofNeurospora intermedia. Genetics 135:993–1002

    PubMed  Google Scholar 

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Communicated by H. Bertrand

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Yuewang, W., Yang, X. & Griffiths, A.J.F. Structure of aGelasinospora linear plasmid closely related to the kalilo plasmid ofNeurospora intermedia . Curr Genet 29, 150–158 (1996). https://doi.org/10.1007/BF02221579

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