Abstract
Cultivated sugarcane clones (Saccharum spp., 2n=100 to 130) are derived from complex interspecific hybridizations between the speciesS. officinarum andS. spontaneum. Using comparative genomic DNA in situ hybridization, we demonstrated that it is possible to distinguish the chromosomes contributed by these two species in an interspecific F1 hybrid and a cultivated clone, R570. In the interspecific F1 studied, we observed n+n transmission of the parental chromosomes instead of the peculiar 2n+n transmission usually described in such crosses. Among the chromosomes of cultivar R570 (2n=107–115) about 10% were identified as originating fromS. spontaneum and about 10% were identified as recombinant chromosomes between the two speciesS. officinarum andS. spontaneum. This demonstrated for the first time the occurrence of recombination between the chromosomes of these two species. The rDNA sites were located by in situ hybridization in these two species and the cultivar R570. This supported different basic chromosome numbers and chromosome structural differences between the two species and provided a first bridge between physical and genetical mapping in sugarcane.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arceneaux G (1965) Cultivated sugarcanes of the world and their botanical derivation. Proc Int Soc Sugar Cane Technol 12:844–854
Bhat SR, Gill BS (1985) The implication of 2n egg gametes in nobilisation and breeding of sugarcane. Euphytica 34:377–384
Bennett ST, Kenton AY, Bennett MD (1992) Genomic in situ hybridization reveals the allopolyploid nature ofMilium montianum (Gramineae). Chromosoma. 101:420–424
Berding N, Roach BT (1987) Germplasm collection, maintenance, and use. In Heinz DJ (ed)Sugarcane improvement through breeding. Elsevier, Amsterdam, pp 143–210
Bremer G (1922) Een cytologisch onderzoek van eenige soorten en soortsbastaarden van het geslachtSaccharum. Arch Suikerindust Nederl Indie Meded Proefsta Java Suikerindust 1–112. (English translation in Genetica 5:97–148; 273–326)
Bremer G (1961) Problems in breeding and cytology of sugar cane. Euphytica 10:59–78
Daniels J, Roach BT (1987) Taxonomy and evolution. In: Heinz DJ (ed) sugarcane improvement through breeding. Elsevier, Amsterdam, pp 7–84
Da Silva J, Honeycutt R, Burnquist W. Al-Janabi S, Sorrells M, Tanksley S, Sobral B (1995)Saccharum spontaneum L. SES 208′ genetic linkage map combining RFLP and PCR-based markers. Mol Breeding 1:165–179
D'Hont A, Lu YH, Gonzalez de Leon D, Grivet L, Feldmann P, Lanaud C, Glaszmann JC (1994) A molecular approach to unravelling the genetics of sugarcane, a complex polyploid of the Andropogoneae tribe. Genome 37:222–230
D'Hont A, Rao P, Feldmann P, Grivet L, Islam-Faridi N, Berding N, Glaszmann JC (1995) Identification and characterisation of intergeneric hybrids,Saccharum officinarum × Erianthus arundinaceus, with molecular markers and in situ hybridiation Theor Appl Genet 91:320–326
Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885
Glaszmann JC, Fautret A, Noyer JL, Feldmann P, Lanaud C (1989) Biochemical genetic markers in sugarcane. Theor Appl Genet 78:537–543
Glaszmann JC, Lu YH, Lanaud C (1990) Variation of nuclear ribosomal DNA in sugarcane. J. Genet Breeding 44:191–198
Grivet L, D'Hont A, Dufour P, Hamon P, Roques D, Glaszmann JC (1994) Comparative genome mapping of sugar cane with other species within theAndropogoneae tribe. Heredity 73:500–508
Grivet L, D'Hont A, Roques D, Feldmann P, Glaszmann JC (1995) RFLP mapping in cultivated sugarcane (Saccharum spp.): genome organization in a highly polyploid and aneuploid interspecific hybrid. Genetics, in press
Gustafson JP, Dillé JE (1992) Chromosome location ofOryza sativa recombination linkage groups. Proc Natl Acad Sci USA 89:8646–8650
Heslop-Harrison JS, Schwarzacher T, Leitch AR, Leitch IJ, Anamthawat-Jonsson K, Mosgoller W, Maluszynska J, Shi M (1991) In situ hybridization. Karyobiology Group. John Innes Institute, Norwich, UK
Heslop-Harrison JS, Harrison G, Leich I (1992) Reprobing of DNA:DNA in situ hybridization preparations. Trends Genet 8:372–373
Jiang J, Gill BS (1994) Nonisotopic in situ hybridization and plant genome mapping: the first 10 years. Genome 37:717–725
Kenton A, Parokonny A Gleba Y, Bennett M (1993) Characterisation of theNicotiana tabacum L. genome by molecular cytogenetics. Mol Gen Genet 240:159–169
Leitch I, Heslop-Harrison. JS (1993) Physical mapping of four sites of 5S rDNA sequences and one site of the alpha-amylase-2 gene in barley (Hordeum vulgare). Genome 36:517–523
Lu YH, D'Hont A, Walker DIT, Rao PS, Feldmann P, Glaszmann J.C (1994a) Relationships among ancestral species of sugarcane revealed with RFLP using single copy maize nuclear probes. Euphytica 78:7–18
Lu YH, D'Hont A, Paulet F, Grivet L, Arnaud M, Glaszmann JC (1994b) Molecular diversity and genome structure in modern sugarcane varieties. Euphytica 78:217–226
Maluszynska J, Heslop-Harrison JS (1993) Physical mapping of the rDNA loci inBrassica species. Genome 36:774–781
Mukai Y, Nakahara Y, Yamamoto M (1993) Simultaneous discrimination of the three genomes in the hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome 36:489–494
Panje R, Babu C (1960) Studies inSaccharum spontaneum. Distribution and geographical association of chromosome numbers. Cytologia 25:152–172
Price S (1961) Cytological studies inSaccharum and allied genera. VII. Maternal chromosome transmission byS. officinarum in intra- and interspecific crosses. Bot Gaz 124:186–190
Price S (1963) Cytogenetics of modern sugar canes. Economic Botany 17:97–105
Price S (1965) Interspecific hybridization in sugarcane breeding. Proc Int Soc Sugar Cane Technol 12:1021–1026
Rayburn A, Gill B (1985) Use of biotin-labeled probes to map specific DNA sequences on wheat chromosomes. J. Hered. 76:78–81
Roach BT (1969) Cytological studies inSaccharum. Chromosome transmission in interspecific and intergeneric crosses. Proc Int Soc Sugar Cane Technol 13:901–920
Roach BT (1972) Nobilisation of sugarcane. Proc Int Soc Sugar Cane Technol 14:206–216
Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann Bot 64:315–324
Schwarzacher T, Anamthawat-Jonsson K, Harrisson G, Islam A, Jian J, Leitch A, Miller T, Rogers W, Heslop-Harrison JS (1992a) Genomic in situ hybridization to identify alien chromosomes and chromosomes segments in wheat. Theor Appl Genet 84:778–786
Simmonds NW (1976) Sugarcanes. In Simmonds NW (ed)Evolution of crop plants. Longman, London, 104–108
Sreenivasan TV, Ahloowalia BS, Heinz DJ, (1987) Cytogenetics. In: Heinz DJ (ed) Sugarcane improvement through breeding. Elsevier, Amsterdam, pp 211–253
Thomas HM, Morgan W, Meredith M, Humphreys M, Thomas H, Leggett J (1994) Identification of parental and recombined chromosomes in hybrid derivatives ofLolium multiflorum ×Festuca pratensis by genomic in situ hybridization. Theor Appl Genet 88:909–913
Author information
Authors and Affiliations
Additional information
Communicated by G. Melchers
Rights and permissions
About this article
Cite this article
D'Hont, A., Grivet, L., Feldmann, P. et al. Characterisation of the double genome structure of modern sugarcane cultivars (Saccharum spp.) by molecular cytogenetics. Molec. Gen. Genet. 250, 405–413 (1996). https://doi.org/10.1007/BF02174028
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02174028