Summary
The sequence of the small-subunit rRNA from the thermoacidophilic archaebacteriumSulfolobus solfataricus has been determined and compared with its counterparts from halophilic and methanogenic archaebacteria, eukaryotes, and eubacteria. TheS. solfataricus sequence is specifically related to those of the other archaebacteria, to the exclusion of the eukaryotic and eubacterial sequences, when examined either by evolutionary distance matrix analyses or by the criterion of minimum change (maximum parsimony). The archaebacterial 16S rRNA sequences all conform to a common secondary structure, with theS. solfataricus structure containing a higher proportion of canonical base pairs and fewer helical irregularities than the rRNAs from the mesophilic archaebacteria.S. solfataricus is unusual in that its 16S rRNA-23S rRNA intergenic spacer lacks a tRNA gene.
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Biggin MD, Gibson TJ, Hong GF (1983) Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci USA 80:3963–3965
Brock TD (1981) Extreme thermophiles of the generaThermus andSulfolobus. In: Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG (eds) The prokaryotes: a handbook on habits, isolation, and identification of bacteria, vol I. Springer-Verlag, New York, pp 978–984
Brosius J, Dull TJ, Noller HF (1980) Complete nucleotide sequence of a 23S ribosomal RNA gene fromEscherichia coli. Proc Natl Acad Sci USA 77:201–204
Brosius J, Palmer JL, Kennedy JP, Noller HF (1978) Complete nucleotide sequence of a 16S ribosomal RNA gene fromEscherichia coli. Proc Natl Acad Sci USA 75:4801–4805
Carbon P, Ebel JP, Ehresmann C (1981) The sequence of the ribosomal 16S RNA fromProteus vulgaris. Nucleic Acids Res 9:2325–2333
Chan Y-L, Gutell R, Noller HF, Wool IG (1984) The nucleotide sequence of a rat 18S ribosomal ribonucleic acid gene and a proposal for the secondary structure of 18S ribosomal ribonucleic acid. J Biol Chem 259:224–230
Dron M, Rahire M, Rochaix J-D (1982) Sequence of the chloroplast 16S rRNA gene and its surrounding regions ofChlamydomonas reinhardii. Nucleic Acids Res 10:7609–7619
Felsenstein J (1978) Cases in which parsimony or compatibility methods will be positively misleading. Syst Zool 27:401–410
Fitch WM (1976) The molecular evolution of cytochrome c in eukaryotes. J Mol Evol 8:13–40
Fox GE, Stackebrandt E, Hespell RBM, Gibson J, Maniloff J, Dyer TA, Wolfe RS, Balch WE, Tanner R, Magrum LJ, Zablen LB, Blakemore R, Gupta R, Bonen L, Lewis BJ, Stahl DL, Luehrsen KR, Chen KN, Woese CR (1980) The phylogeny of prokaryotes. Science 209:457–463
Fox GE, Luehrsen KR, Woese CR (1982) Archaebacterial 5S ribosomal RNA. Zentralbl Bakteriol Mikrobiol Hyg [C] 3: 330–345
Graf L, Roux E, Stutz E, Kossel H (1982) Nucleotide sequence of aEuglena gracilis chloroplast gene coding for the 16S rRNA: homologies toE. coli andZea mays chloroplast rRNA. Nucleic Acids Res 10:6369–6381
Gupta R, Lanter JM, Woese CR (1983) Sequence of the 16S ribosomal RNA fromHalobacterium volcanii, an archaebacterium. Science 221:656–659
Jarsch M, Bock A (1985) Sequence of the 16S ribosomal RNA gene fromMethanococcus vannielii: evolutionary implications. Syst Appl Microbiol 6:54–59
Lake JA, Henderson E, Oakes M, Clark MW (1984) Eocytes: A new ribosome structure indicates a kingdom with a close relationship to eukaryotes. Proc Natl Acad Sci USA 81:3786–3790
Lake JA, Clark MW, Henderson E, Fay SP, Oakes M, Scheinman A, Thornber JP, Mah RA (1985) Eubacteria, halobacteria, and the origin of photosynthesis: the photocytes. Proc Natl Acad Sci USA 82:3716–3720
Leffers H, Garrett RA (1984) The nucleotide sequence of the 16S ribosomal RNA gene of the archaebacteriumHalococcus morrhuae. EMBO 3:1613–1619
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 1–435
Mankin AS, Kopylov AM, Bogdanov AA (1981) Modification of 18S rRNA in the 40S ribosomal subunit with dimethyl sulfate. FEBS Lett 134:11–14
McCarroll R, Olsen GJ, Stahl UD, Woese CR, Sogin ML (1983) Nucleotide sequence ofDictyostelium discoideum small-subunit ribosomal ribonucleic acid inferred from the gene sequence: evolutionary implications. Biochemistry 22:5858–5868
Messing J, Vieira J (1982) A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:169–176
Messing J, Carlson J, Hagen G, Rubenstein I, Oleson A (1984) Cloning and sequencing of the ribosomal RNA genes in maize: the 17S region. DNA 3:31–40
Nelles L, Fang B-L, Volckaert G, Vandenberghe A, DeWachter R (1984) Nucleotide sequence of a crustacean 18S ribosomal RNA gene and secondary structure of eukaryotic small subunit ribosomal RNAs. Nucleic Acids Res 12:8749–8768
Olsen GJ (1983) Comparative analysis of nucleotide sequence data PhD thesis, University of Colorado, Denver, pp 16–28
Ozaki T, Hoshikawa Y, Iida Y, Iwabuchi M (1984) Sequence analysis of the transcribed and 5′ non-transcribed regions of the ribosomal RNA gene inDictyostelium discoideum. Nucleic Acids Res 12:4171–4184
Raynal F, Michot B, Bachellerie J-P (1984) Complete nucleotide sequence of mouse 18S rRNA gene: comparison with other available homologs. FEBS Lett 167:263–268
Rubtsov PM, Musakhanov MM, Zakharyev VM, Krayev AS, Skryabin KG, Bayev AA (1980) The structure of the yeast ribosomal RNA genes. I. The complete sequence of the 18S ribosomal RNA gene fromSaccharomyces cerevisiae. Nucleic Acids Res 8:5779–5794
Salim M, Maden BEH (1981) Nucleotide sequence ofXenopus laevis 18S ribosomal RNA inferred from gene sequence. Nature 291:205–208
Schwarz Z, Kossel H (1980) The primary structure of 16S rDNA fromZea mays chloroplast is homologous toE. coli 16S rRNA. Nature 283:739–742
Stahl DA, Luehrsen KR, Woese CR, Pace NR (1981) An unusual 5S rRNA, fromSulfolobus acidocaldarius, and its implications for a general 5S rRNA structure. Nucleic Acids Res 9:6129–6137
Stanier RY (1970) Some aspects of the biology of cells and their possible evolutionary significance. Symp Soc Gen Microbiol 20:1–38
Stöffler-Meilicke M, Böhme C, Strobel O, Böck A, Stöffler G (1985) An evaluation of ribosomal morphology as a phylogenetic marker. Science, in press
Takaiwa F, Oono D, Sugiura M (1984) The complete nucleotide sequence of a rice 17S rRNA gene. Nucleic Acids Res 12:5441–5448
Tomioka N, Sugiura M (1983) The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga,Anacystis nidulans. Mol Gen Genet 191:46–50
Torczynski R, Bollon AP, Fuke M (1983) The complete nucleotide sequence of the rat 18S ribosomal RNA gene and comparison with the respective yeast and frog genes. Nucleic Acids Res 11:4879–4890
Woese CR, Fox GE (1977) Phylogenetic structure of the prokaryotic domain: the primary kingdoms. Proc Natl Acad Sci USA 74:5088–5099
Woese CR, Wolfe RS (eds) (1985) The bacteria, vol 8: Archaebacteria. Academic Press, New York, pp 1–582
Woese CR, Gutell RR, Gupta R, Noller HF (1983) Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. Microbiol Rev 47:621–669
Woese CR, Gupta R, Hahn CM, Zillig W, Tu J (1984) The phylogenetic relationships of three sulfur-dependent archaebacteria. Syst Appl Microbiol 5:97–105
Yang DC, Kaine BP, Woese CR (1985) The phylogeny of archaebacteria. Syst Appl Microbiol, in press
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Olsen, G.J., Pace, N.R., Nuell, M. et al. Sequence of the 16S rRNA gene from the thermoacidophilic archaebacteriumSulfolobus solfataricus and its evolutionary implications. J Mol Evol 22, 301–307 (1985). https://doi.org/10.1007/BF02115685
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DOI: https://doi.org/10.1007/BF02115685