Skip to main content
Log in

Reduction of abscisic acid content and induction of sprouting in potato,Solanum tuberosum L., by thidiazuron

  • Published:
Journal of Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The effect of [(N-phenyl-N′-1,2,3-thidiazol-5-ylurea)] (thidazuron) on sprouting of potato (Solanum tuberosum L.) tubers and the role of ABA in bud break and subsequent bud growth were studied. Abscisic acid (ABA) was quantitated by enzyme-linked immunosorbent assay (ELISA) from the peel of potato tubers. The ELISA results were also validated by gas chromatography-electron capture detector and confirmed by gas chromatography-mass spectrometry and by a lettuce hypocotyl bioassay. The degree of rest in the tubers was associated with ABA content in the peel. Basal portion (where tuber was attached to mother plant) contained the highest amount of ABA. Thidiazuron reduced ABA content and induced potato tuber sprouting. Exogenously applied ABA stimulated growth of buds that had emerged from dormancy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bakken TJ, Boe AA (1982) Two bioassay techniques for determining abscisic concentration. J Am Soc Hortic Sci 107:109–112

    Google Scholar 

  • Boo L (1961) The effect of gibberellic acid on the inhibitor-β complex in resting potato. Physiol Plant 14:676–681

    Google Scholar 

  • Capelle SC, Mok DWS, Kirchner SC, Mok MC (1983) Effects of thidiazuron on cytokinin autonomy and the metabolism of N6-(Δ2-isopentenyl) [8-14C]adenosine in callus tissue ofPhaseolus lunatus L. Plant Physiol 73:796–802

    Google Scholar 

  • Fales HN, Jaouni TM, Babashak JF (1973) Simple device for preparing etheral diazomethane without resorting to codistillation. Anal Chem 45:2302–2303

    Google Scholar 

  • Hawthorne WI (1969) The effect of chemicals and other treatments on the rest period of some horticultural crops. Dissertation Abstr Int, 2978-B, No. 70-245

  • Hemberg T (1967) The action of endogenous growth-inhibiting substances and gibberellins on the rest period of the potato tuber. Wissensch Univ Rostock 45:661–666

    Google Scholar 

  • Hemberg T (1970) The action of some cytokinins on the rest period and the content of acid growth-inhibiting substances in potato. Physiol Plant 23:850–858

    Google Scholar 

  • Leroux B, Maldiney R, Miginiac E, Sossountzov L, Sotta B (1985) Comparative quantitation of abscisic acid in plant extracts by gas-liquid chromatography and an enzyme-linked immunosorbent assay using the avidin biotin system. Planta 166:524–529.

    Google Scholar 

  • Letham DS (1978) Naturally-occurring plant growth regulators other than the principal hormones of higher plants. In: Letham DS, Goodwin PB, Higgins TJV (eds) Phytohormones and related compounds: A comprehensive treatise, Vol. I, Biochemistry of phytohormones and related compounds. Elsevier/North Holland, Amsterdam, pp 349–465

    Google Scholar 

  • Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y, Okamoto T (1982) Cytokinin activity ofN-phenyl-N-1,2,3-thidiazol-5-yulrea (thidiazuron). Phytochemistry 21:1509–1511

    Google Scholar 

  • Obhilidalova L, Slaby K, Sebanek J (1980) Changes in endogenous cytokinins and gibberellins during storage of potato tubers. Hort Abstr 6:1352

    Google Scholar 

  • Phytodeteck (1985) Enzyme immunoassay test for the quantitative determination of plant hormones. Idetek, Inc., San Bruno, CA

    Google Scholar 

  • Powell LE, Maybee C (1985) Hormone analysis employing ‘BAKER’-10 SPE™ disposable columns. Plant Physiol 77:S-75

    Google Scholar 

  • Rappaport L, Blumenthal-Goldschmidt S, Clegg MD, Smith OE (1965) Regulation of bud rest in tubers of potato,Solanum tuberosum L. 1. Effect of growth substances on excised potato buds. Plant Cell Physiol 6:587–599

    Google Scholar 

  • Rivier L, Milon H, Pilet PE (1977) Gas chromatography-mass spectrometric determinations of abscisic acid levels in the cap and the apex of maize roots. Planta 134:23–27

    Google Scholar 

  • Thomas JC, Katterman FR (1986) Cytokinin activity induced by thidiazuron. Plant Physiol 81:681–683

    Google Scholar 

  • Thomas TH (1981) Hormonal control of dormancy in relation to post-harvest horticulture. Ann Appl Biol 98:531–538

    Google Scholar 

  • Thomas TH, Wurr DCE (1976) Gibberellin and growth inhibitor changes in potato tuber buds in response to cold treatment. Ann Appl Biol 83:317–320

    Google Scholar 

  • Tsukamoto Y (1972) Breaking dormancy in the gladiolus corm with cytokinins. Proc Jpn Acad 48:34–38

    Google Scholar 

  • Wang SY, Steffens GL, Faust M (1986) Breaking bud dormancy in apple with a plant bioregulator, thidiazuron. Phytochemistry 25:311–317

    Google Scholar 

  • Wang SY, Ji ZL, Sun T, Faust M (1987) Effect of thidiazuron of abscisic acid content in apple bud relative to dormancy. Physiol Plant 71:105–109

    Google Scholar 

  • Weiler EW (1982) An enzyme-immunoassay for cis-(+)-abscisic acid. Physiol Plant 54:510–514

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

On leave from the Department of Horticulture, South China Agricultural University, Guangzhou, People's Republic of China.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ji, Z.L., Wang, S.Y. Reduction of abscisic acid content and induction of sprouting in potato,Solanum tuberosum L., by thidiazuron. J Plant Growth Regul 7, 37–44 (1988). https://doi.org/10.1007/BF02054160

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02054160

Keywords

Navigation