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Nerine latent virus: some properties and serological detectability in Nerine bowdenii

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Abstract

Nerine latent virus (NeLV), first found inNerine bowdenii, may occur also in the otherNerine species investigated so far:N. sarniensis, N. flexuosa ‘Alba’, andN. ‘Mansellii’.

Chenopodium amaranticolor, C. quinoa, andGomphrena globosa sometimes reacted with local lesions after mechanical inoculation with NeLV.Nicotiana clevelandii andHippeastrum were symptomless hosts. In this respect NeLV resembled the incompletely describedHippeastrum latent virus (HLV).

Serologically NeLV was closely related to HLV and to carnation latent virus (CaLV), but differed from the latter in host plant reaction. A more distant relationship was observed with some other carlaviruses, wheareas NeLV also reacted with an antiserum to potato virus X.

Depending on the lot, NeLV could be detected rather reliably with the micro-precipitin test inN. bowdenii ‘Van Roon’, but less well in ‘63’. Better results were obtained with the microplate method of enzyme-linked immunosorbent assay (ELISA).

The average particle length was 664 nm, the sedimentation coefficient 155 S and the buoyant density 1.298 g/cm3.

NeLV can be considered as a member of the carlavirus group. On basis of priority HLV may be considered as NeLV.

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Maat, D.Z., Huttinga, H. & Hakkaart, F.A. Nerine latent virus: some properties and serological detectability in Nerine bowdenii. Netherlands Journal of Plant Pathology 84, 47–59 (1978). https://doi.org/10.1007/BF01976408

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