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pBINPLUS: An improved plant transformation vector based on pBIN19

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Abstract

We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number inE. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.

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van Engelen, F.A., Molthoff, J.W., Conner, A.J. et al. pBINPLUS: An improved plant transformation vector based on pBIN19. Transgenic Research 4, 288–290 (1995). https://doi.org/10.1007/BF01969123

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  • DOI: https://doi.org/10.1007/BF01969123

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