Abstract
In order to investigate the humoral response to tuberculosis in different categories of patients, serum antibody levels to six epitopes ofMycobacterium tuberculosis in adult pulmonary and child tuberculosis were determined. Serum antibody titres were determined by competitive inhibition with radio-labelled murine monoclonal antibodies in 67 adults and 85 children with tuberculosis and in 79 age-matched controls. BCG vaccination (n=39) and self-healed tuberculosis (n=11) in adults gave rise to higher antibody titres to TB68, TB23 and TB72 epitopes (all p<0.003) when compared to non-vaccinated controls (n=18). TB68 titres were higher (p=0.006) in self-healed than in vaccinated adults. Adult sputum-negative patients (n=15) had higher titres to TB71 (p=0.015) and ML34 (p=0.02) epitopes compared to BCG-vaccinated healthy controls, while sputum-positive patients (n=41) had higher titres to all epitopes tested (all p<10−4). The diagnostic sensitivity, with a 95 % specificity, was best with the combination of probes TB23, TB68, TB72 for sputum-positive (85 %) and TB78, ML34 (53 %) for sputum-negative patients. Antibody titres in children with tuberculosis were lower than in adult patients; diagnostic sensitivity in histologically or microbiologically proven cases (n=18) was only 44 %, while that in mediastinal lymphadenitis (n=67) was 13.5 %. This study suggests that the magnitude and specificity of the humoral response to tubercle bacilli varies with site and severity of infection; the implications for pathogenesis or protective immunity are discussed.
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Comstock, G. W., Daniel, T. M., Snider, D. E., Edwards, P.A., Hopewell, P. C., MacVandiviere, H. The tuberculin skin test. American Review of Respiratory Diseases 1981, 124:356–363.
Coates, A. R. M., Hewitt, J., Allen, B. W., Ivanyi, J., Mitchison, D. A. Antigenic diversity ofMycobacterium tuberculosis andMycobacterium bovis detected by means of monoclonal antibodies. Lancet 1981, ii: 167–169.
Ivanyi, J., Morris, J. A., Keen, M. Studies with monoclonal antibodies to mycobacteria. In: Macario, A. J. L., Conway de Macario, E. (ed.): Monoclonal antibodies against bacteria. Academic Press, New York, 1985, p. 59–90.
Engers, H. D., Houba, V., Bennedsen, J., Buchanan, T. M., Chaparas, S. D., Kadival, G., Closs, O., David, J. R., van Embden, J. D. A., Godal, T., Mustafa, S. A., Ivanyi, J., Young, D. B., Kaufmann, S. H. E., Khomenko, A. G., Kolk, A. H. J., Kubin, M., Louis, J. A., Minden, P., Shinnick, T. M., Trnka, L., Young, R. A. Results of a World Health Organization sponsored workshop to characterise antigens recognized by mycobacteria-specific monoclonal antibodies. Infection and Immunity 1986, 51: 718–720.
Hewitt, J., Coates, A. R. M., Mitchison, D. A., Ivanyi, J. The use of murine monoclonal antibodies without purification of antigen in the serodiagnosis of tuberculosis. Journal of Immunological Methods 1982, 55: 205–211.
Ivanyi, J., Krambovitis, E., Keen, M. Evaluation of a monoclonal antibody (TB72) based serological test for tuberculosis. Clinical and Experimental Immunology 1983, 54: 337–345.
Hoeppner, V., Jackett, P. S., Swanson Beck, J., Kardjito, T., Grange, J. M., Ivanyi, J. Appraisal of the monoclonal antibody-based competition test for the serology of tuberculosis in Indonesia. Serodiagnosis and Immunotherapy 1987, 1: 69–77.
Miller, F. J. W. Tuberculosis in children. Churchill Livingstone, Edinburgh, 1981, p. 3–18.
Friedman, E., Silverman, F. Use of BCG vaccine as a new diagnostic test for tuberculosis. Pediatrics 1952, 9: 280–285.
Udani, P. M. BCG test in the diagnosis of tuberculosis in children. Indian Pediatrics 1982, 19: 563–581.
Nassau, E., Parson, E. R., Johnson, C. D. Detection of antibodies toMycobacterium tuberculosis by solid phase radioimmunoassay. Journal of Immunological Methods 1975, 6: 261–271.
Armitage, P., Berry, G. Statistical methods in medical research. Blackwell Scientific Publications, Oxford, 1987.
Young, D. B., Ivanyi, J., Cox, J. H., Lamb, J. R. The 65 kDa antigen of mycobacteria - a common bacterial protein. Immunology Today 1987, 8: 215–219.
Hunter, S. W., Gaylord, H., Brennan, P. J. Structure and antigenicity of the phosphorylated lipopolysaccharide antigens from the leprosy and tubercle bacilli. Journal of Biological Chemistry 1986, 261: 12345–12351.
Kaplan, M. H., Chase, M. W. Antibodies to mycobacteria in human tuberculosis. I: Development of antibodies before and after antimicrobial therapy. Journal of Infectious Diseases 1980, 142: 825–834.
Melsom, M. D. Serodiagnosis of leprosy: the past, the present, and some prospect for the future. International Journal of Leprosy 1983, 51: 235–252.
Douvas, G. S., Crowle, A. J. Mechanisms involved in the antibody-mediated suppresion of tuberculin-type delayed hypersensitivity: the effects of antigen concentration on tolerance induction, the ability of antigen-antibody complexes to tolerize and the effects of tolerization on antigen-induced in vitro proliferation. Annales d'Immunologie 1981, 132 C: 307–318.
Milon, G., Marchal, G., Seman, M., Truffa-Bachi, P., Zilberfarb, V. Is the delayed-type hypersensitivity observed after a low dose of antigen mediated by T helper cells. Journal of Immunology 1983, 130: 1103–1107.
LeClerq, S. A., Bretscher, P. A. T cells expressing delayed type-hypersensitivity can be derived from a humorally immune lymphocyte population. European Journal of Immunology 1987, 17: 949–954.
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Bothamley, G., Udani, P., Rudd, R. et al. Humoral response to defined epitopes of tubercle bacilli in adult pulmonary and child tuberculosis. Eur. J. Clin. Microbiol. Infect. Dis. 7, 639–645 (1988). https://doi.org/10.1007/BF01964242
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DOI: https://doi.org/10.1007/BF01964242