Abstract
The cytotoxic effect caused by the hypomethylating agent S-adenosyl-L-homocysteine (SAH) was compared with that of two drugs commonly used to induce DNA hypomethylation, 5-azacytidine and 5-aza-2′-deocycytidine. Two in vitro cytotoxicity tests, the tetrazolium MTT assay and the intracellular lactate dehydrogenase (LDH) activity test, suggest that SAH induces hypomethylation without causing any cytotoxic effect. We propose the use of SAH as a non-cytotoxic agent which may be more suitable for inducing experimental DNA hypomethylation.
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Razin, A., and Cedar, H., Microb. Rev.55 (1991) 451.
Lewis, J., and Bird, A., FEBS Lett.2 (1991) 155.
Jones, P. A., and Taylor, S. M., Nucl. Acids Res.9, 12 (1981) 2933.
Landolph, J. R., and Jones, P. A., Cancer Res.42 (1982) 817.
Michalowsky, L., and Jones, P. A., Molec. Cell Biol.7 (1987) 3076.
Davidson, S., Crowther, P., Radley, J., and Woodcock, D., Eur. J. Cancer28, No. 2/3 (1992) 362.
Barbés, C., Sánchez, J., Yebra, M. J., Robert-Geró, M., and Hardisson, C., FEMS Microbiol. Lett.69 (1990) 239.
De Cabo, S. F., Sentís, C., and Fernández-Piqueras, J., Histochemistry97 (1992) 499.
Ponsoda, X., Jover, R., Castell, J. V., and Gómez-Lechón, M. J., J. Tiss. Cult. Meth.13 (1991) 21.
Jones, P. A., and Taylor, S. M., Cell20 (1980) 85.
Vassault, A., in: Methods of Enzymatic Analysis. p. 118. Eds H. V. Bergmeyer, J. Bergmeyer and M. Grabl. Weinheim FRG Verlag Chemie, Weinheim (Germany) 1983.
Edmondson, J. M., Armstrong, L. S., and Martínez, A. O., J. Tiss. Cult. Meth.11 (1988) 15.
Mosmann, T., J. Immun. Meth.65 (1983) 55.
Borenfreund, D., Babich, H., and Martin-Alguacil, N., Toxic. in Vitro2 (1988) 1.
Ueland, P. M., Pharmac. Rev.34 (1982) 223.
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De Cabo, S.F., Hazen, M.J., Molero, M.L. et al. S-adenosyl-L-homocysteine: A non-cytotoxic hypomethylating agent. Experientia 50, 658–659 (1994). https://doi.org/10.1007/BF01952867
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DOI: https://doi.org/10.1007/BF01952867